RAPID SOUTHERN BLOT PROTOCOL FOR THE ANALYSIS OF TARGETED MUTAGENESIS IN THE MOUSE IGHG1 LOCUS

RAPID SOUTHERN BLOT PROTOCOL FOR THE ANALYSIS OF TARGETED MUTAGENESIS IN THE MOUSE IGHG1 LOCUS

The efficiency of homologous recombination relies on the lenght of the homology arms and the chromatin compactation of the locus. In ES cells the immuoglobulin locu is compacted. Thus, the homologous recombination events occur at a very low frequency. A high number of ES cell colonies have to be ana...

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Bibliographic Details
Main Authors:	ALENA A. BENCSIK, I. BENCSIK, JANA STĂNCULEȚ, M. BURA
Format:	Article
Language:	English
Published:	Agroprint Timisoara 2023-11-01
Series:	Scientific Papers Animal Science and Biotechnologies
Subjects:	ighg1 gene immunoglobulin southern blot embryonic stem cells gene targeting
Online Access:	https://spasb.ro/index.php/public_html/article/view/1654

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