Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01

CRISPR/Cas9-mediated homology-directed recombination is an efficient method to express target genes. Based on the above method, providing ideal neutral integration sites can ensure the reliable, stable, and high expression of target genes. In this study, we obtained a fluorescent transformant with n...

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Bibliographic Details
Main Authors: Sheng Tong, Kexin An, Wuxi Chen, Mengdan Chai, Yuanxia Sun, Qinhong Wang, Demao Li
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2023-03-01
Series:Synthetic and Systems Biotechnology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X22001259