The antimicrobial peptide Magainin-2 interacts with BamA impairing folding of E. coli membrane proteins
Antimicrobial peptides (AMPs) are a unique and diverse group of molecules endowed with a broad spectrum of antibiotics properties that are being considered as new alternative therapeutic agents. Most of these peptides are membrane-active molecules, killing bacteria by membrane disruption. However, r...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2022.1013788/full |
| _version_ | 1798030228734345216 |
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| author | Angela Di Somma Angela Di Somma Carolina Cané Antonio Moretta Anna Illiano Anna Illiano Gabriella Pinto Gabriella Pinto Domenico Cavasso Angela Amoresano Angela Amoresano Luigi Paduano Angela Duilio Angela Duilio |
| author_facet | Angela Di Somma Angela Di Somma Carolina Cané Antonio Moretta Anna Illiano Anna Illiano Gabriella Pinto Gabriella Pinto Domenico Cavasso Angela Amoresano Angela Amoresano Luigi Paduano Angela Duilio Angela Duilio |
| author_sort | Angela Di Somma |
| collection | DOAJ |
| description | Antimicrobial peptides (AMPs) are a unique and diverse group of molecules endowed with a broad spectrum of antibiotics properties that are being considered as new alternative therapeutic agents. Most of these peptides are membrane-active molecules, killing bacteria by membrane disruption. However, recently an increasing number of AMPs was shown to enter bacterial cells and target intracellular processes fundamental for bacterial life. In this paper we investigated the mechanism of action of Maganin-2 (Mag-2), a well-known antimicrobial peptide isolated from the African clawed frog Xenopus laevis, by functional proteomic approaches. Several proteins belonging to E. coli macromolecular membrane complexes were identified as Mag-2 putative interactors. Among these, we focused our attention on BamA a membrane protein belonging to the BAM complex responsible for the folding and insertion of nascent β-barrel Outer Membrane Proteins (OMPs) in the outer membrane. In silico predictions by molecular modelling, in vitro fluorescence binding and Light Scattering experiments carried out using a recombinant form of BamA confirmed the formation of a stable Mag-2/BamA complex and indicated a high affinity of the peptide for BamA. Functional implications of this interactions were investigated by two alternative and complementary approaches. The amount of outer membrane proteins OmpA and OmpF produced in E. coli following Mag-2 incubation were evaluated by both western blot analysis and quantitative tandem mass spectrometry in Multiple Reaction Monitoring scan mode. In both experiments a gradual decrease in outer membrane proteins production with time was observed as a consequence of Mag-2 treatment. These results suggested BamA as a possible good target for the rational design of new antibiotics since this protein is responsible for a crucial biological event of bacterial life and is absent in humans. |
| first_indexed | 2024-04-11T19:37:57Z |
| format | Article |
| id | doaj.art-fe921602330c4e1da61fe9f0e31c8e42 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-04-11T19:37:57Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-fe921602330c4e1da61fe9f0e31c8e422022-12-22T04:06:48ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462022-10-011010.3389/fchem.2022.10137881013788The antimicrobial peptide Magainin-2 interacts with BamA impairing folding of E. coli membrane proteinsAngela Di Somma0Angela Di Somma1Carolina Cané2Antonio Moretta3Anna Illiano4Anna Illiano5Gabriella Pinto6Gabriella Pinto7Domenico Cavasso8Angela Amoresano9Angela Amoresano10Luigi Paduano11Angela Duilio12Angela Duilio13Department of Chemical Sciences, University of Naples “Federico II”, Naples, ItalyNational Institute of Biostructures and Biosystems (INBB), Rome, ItalyDepartment of Chemical Sciences, University of Naples “Federico II”, Naples, ItalyInstitut NeuroMyoGène, Unité Physiopathologie et Génétique du Neurone et du Muscle, CNRS UMR5261, INSERM U1315, Université Claude Bernard Lyon 1, Lyon, FranceDepartment of Chemical Sciences, University of Naples “Federico II”, Naples, ItalyNational Institute of Biostructures and Biosystems (INBB), Rome, ItalyDepartment of Chemical Sciences, University of Naples “Federico II”, Naples, ItalyNational Institute of Biostructures and Biosystems (INBB), Rome, ItalyDepartment of Chemical Sciences, University of Naples “Federico II”, Naples, ItalyDepartment of Chemical Sciences, University of Naples “Federico II”, Naples, ItalyNational Institute of Biostructures and Biosystems (INBB), Rome, ItalyDepartment of Chemical Sciences, University of Naples “Federico II”, Naples, ItalyDepartment of Chemical Sciences, University of Naples “Federico II”, Naples, ItalyNational Institute of Biostructures and Biosystems (INBB), Rome, ItalyAntimicrobial peptides (AMPs) are a unique and diverse group of molecules endowed with a broad spectrum of antibiotics properties that are being considered as new alternative therapeutic agents. Most of these peptides are membrane-active molecules, killing bacteria by membrane disruption. However, recently an increasing number of AMPs was shown to enter bacterial cells and target intracellular processes fundamental for bacterial life. In this paper we investigated the mechanism of action of Maganin-2 (Mag-2), a well-known antimicrobial peptide isolated from the African clawed frog Xenopus laevis, by functional proteomic approaches. Several proteins belonging to E. coli macromolecular membrane complexes were identified as Mag-2 putative interactors. Among these, we focused our attention on BamA a membrane protein belonging to the BAM complex responsible for the folding and insertion of nascent β-barrel Outer Membrane Proteins (OMPs) in the outer membrane. In silico predictions by molecular modelling, in vitro fluorescence binding and Light Scattering experiments carried out using a recombinant form of BamA confirmed the formation of a stable Mag-2/BamA complex and indicated a high affinity of the peptide for BamA. Functional implications of this interactions were investigated by two alternative and complementary approaches. The amount of outer membrane proteins OmpA and OmpF produced in E. coli following Mag-2 incubation were evaluated by both western blot analysis and quantitative tandem mass spectrometry in Multiple Reaction Monitoring scan mode. In both experiments a gradual decrease in outer membrane proteins production with time was observed as a consequence of Mag-2 treatment. These results suggested BamA as a possible good target for the rational design of new antibiotics since this protein is responsible for a crucial biological event of bacterial life and is absent in humans.https://www.frontiersin.org/articles/10.3389/fchem.2022.1013788/fullantimicrobial peptidesproteomicsmembrane proteinsmechanism of actionstructural and functional characterization |
| spellingShingle | Angela Di Somma Angela Di Somma Carolina Cané Antonio Moretta Anna Illiano Anna Illiano Gabriella Pinto Gabriella Pinto Domenico Cavasso Angela Amoresano Angela Amoresano Luigi Paduano Angela Duilio Angela Duilio The antimicrobial peptide Magainin-2 interacts with BamA impairing folding of E. coli membrane proteins Frontiers in Chemistry antimicrobial peptides proteomics membrane proteins mechanism of action structural and functional characterization |
| title | The antimicrobial peptide Magainin-2 interacts with BamA impairing folding of E. coli membrane proteins |
| title_full | The antimicrobial peptide Magainin-2 interacts with BamA impairing folding of E. coli membrane proteins |
| title_fullStr | The antimicrobial peptide Magainin-2 interacts with BamA impairing folding of E. coli membrane proteins |
| title_full_unstemmed | The antimicrobial peptide Magainin-2 interacts with BamA impairing folding of E. coli membrane proteins |
| title_short | The antimicrobial peptide Magainin-2 interacts with BamA impairing folding of E. coli membrane proteins |
| title_sort | antimicrobial peptide magainin 2 interacts with bama impairing folding of e coli membrane proteins |
| topic | antimicrobial peptides proteomics membrane proteins mechanism of action structural and functional characterization |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2022.1013788/full |
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