Imaging and analysis on the interaction between human antigen-pulsed Vδ2 T cells and antigen-specific CD4 T cells

Imaging and analysis on the interaction between human antigen-pulsed Vδ2 T cells and antigen-specific CD4 T cells

Summary: This protocol describes how to visualize surface protein-protein co-localization across a cell-cell interface between antigen-presenting γδ-T cells and CD4 T cells. By consolidating immunofluorescence assay, confocal microscopy and 3D imaging analysis, it enables assessment of interaction b...

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Bibliographic Details
Main Authors: Yufei Mo, Allen Ka Loon Cheung, Yue Liu, Li Liu, Zhiwei Chen
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell culture
Cell isolation
Immunology
Microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672100160X

Internet

http://www.sciencedirect.com/science/article/pii/S266616672100160X

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