Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology.
Elevated concentrations of serum non-esterified fatty acids (NEFA), associated with maternal disorders such as obesity and type II diabetes, alter the ovarian follicular micro-environment and have been associated with subfertility arising from reduced oocyte developmental competence. We have asked w...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3157355?pdf=render |
| _version_ | 1818316761828687872 |
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| author | Veerle Van Hoeck Roger G Sturmey Pablo Bermejo-Alvarez Dimitrios Rizos Alfonso Gutierrez-Adan Henry J Leese Peter E J Bols Jo L M R Leroy |
| author_facet | Veerle Van Hoeck Roger G Sturmey Pablo Bermejo-Alvarez Dimitrios Rizos Alfonso Gutierrez-Adan Henry J Leese Peter E J Bols Jo L M R Leroy |
| author_sort | Veerle Van Hoeck |
| collection | DOAJ |
| description | Elevated concentrations of serum non-esterified fatty acids (NEFA), associated with maternal disorders such as obesity and type II diabetes, alter the ovarian follicular micro-environment and have been associated with subfertility arising from reduced oocyte developmental competence. We have asked whether elevated NEFA concentrations during oocyte maturation affect the development and physiology of zygotes formed from such oocytes, using the cow as a model. The zygotes were grown to blastocysts, which were evaluated for their quality in terms of cell number, apoptosis, expression of key genes, amino acid turnover and oxidative metabolism. Oocyte maturation under elevated NEFA concentrations resulted in blastocysts with significantly lower cell number, increased apoptotic cell ratio and altered mRNA abundance of DNMT3A, IGF2R and SLC2A1. In addition, the blastocysts displayed reduced oxygen, pyruvate and glucose consumption, up-regulated lactate consumption and higher amino acid metabolism. These data indicate that exposure of maturing oocytes to elevated NEFA concentrations has a negative impact on fertility not only through a reduction in oocyte developmental capacity but through compromised early embryo quality, viability and metabolism. |
| first_indexed | 2024-12-13T09:26:34Z |
| format | Article |
| id | doaj.art-feb14bc4829d40c3b0449c31b9b5dd59 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T09:26:34Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-feb14bc4829d40c3b0449c31b9b5dd592022-12-21T23:52:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2318310.1371/journal.pone.0023183Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology.Veerle Van HoeckRoger G SturmeyPablo Bermejo-AlvarezDimitrios RizosAlfonso Gutierrez-AdanHenry J LeesePeter E J BolsJo L M R LeroyElevated concentrations of serum non-esterified fatty acids (NEFA), associated with maternal disorders such as obesity and type II diabetes, alter the ovarian follicular micro-environment and have been associated with subfertility arising from reduced oocyte developmental competence. We have asked whether elevated NEFA concentrations during oocyte maturation affect the development and physiology of zygotes formed from such oocytes, using the cow as a model. The zygotes were grown to blastocysts, which were evaluated for their quality in terms of cell number, apoptosis, expression of key genes, amino acid turnover and oxidative metabolism. Oocyte maturation under elevated NEFA concentrations resulted in blastocysts with significantly lower cell number, increased apoptotic cell ratio and altered mRNA abundance of DNMT3A, IGF2R and SLC2A1. In addition, the blastocysts displayed reduced oxygen, pyruvate and glucose consumption, up-regulated lactate consumption and higher amino acid metabolism. These data indicate that exposure of maturing oocytes to elevated NEFA concentrations has a negative impact on fertility not only through a reduction in oocyte developmental capacity but through compromised early embryo quality, viability and metabolism.http://europepmc.org/articles/PMC3157355?pdf=render |
| spellingShingle | Veerle Van Hoeck Roger G Sturmey Pablo Bermejo-Alvarez Dimitrios Rizos Alfonso Gutierrez-Adan Henry J Leese Peter E J Bols Jo L M R Leroy Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology. PLoS ONE |
| title | Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology. |
| title_full | Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology. |
| title_fullStr | Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology. |
| title_full_unstemmed | Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology. |
| title_short | Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology. |
| title_sort | elevated non esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology |
| url | http://europepmc.org/articles/PMC3157355?pdf=render |
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