Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation
Abstract We present a miniature oblique back-illumination microscope (mOBM) for imaging the microcirculation of human oral mucosa, enabling real-time, label-free phase contrast imaging of individual leukocytes circulating in the bloodstream, as well as their rolling and adhesion on vascular walls—th...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-10-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-45018-1 |
| _version_ | 1797576942233321472 |
|---|---|
| author | Arutyun Bagramyan Charles P. Lin |
| author_facet | Arutyun Bagramyan Charles P. Lin |
| author_sort | Arutyun Bagramyan |
| collection | DOAJ |
| description | Abstract We present a miniature oblique back-illumination microscope (mOBM) for imaging the microcirculation of human oral mucosa, enabling real-time, label-free phase contrast imaging of individual leukocytes circulating in the bloodstream, as well as their rolling and adhesion on vascular walls—the initial steps in leukocyte recruitment that is a hallmark of inflammation. Using the mOBM system, we studied the leukocyte-endothelial interactions in healthy and locally inflamed tissue and observed drastic changes in leukocyte movement (velocity and displacement profile). Our findings suggest that real-time imaging of leukocyte dynamics can provide new diagnostic insights (assessment of inflammation, temporal progression of disease, evaluation of therapeutic response, etc.) that are not available using conventional static parameters such as cell number and morphology. |
| first_indexed | 2024-03-10T22:00:57Z |
| format | Article |
| id | doaj.art-ff2f82ba0ee642e499427ba98c4700fa |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T22:00:57Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ff2f82ba0ee642e499427ba98c4700fa2023-11-19T12:58:21ZengNature PortfolioScientific Reports2045-23222023-10-011311710.1038/s41598-023-45018-1Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculationArutyun Bagramyan0Charles P. Lin1Wellman Center for Photomedicine and Center for Systems Biology, Massachusetts General Hospital, Harvard Medical SchoolWellman Center for Photomedicine and Center for Systems Biology, Massachusetts General Hospital, Harvard Medical SchoolAbstract We present a miniature oblique back-illumination microscope (mOBM) for imaging the microcirculation of human oral mucosa, enabling real-time, label-free phase contrast imaging of individual leukocytes circulating in the bloodstream, as well as their rolling and adhesion on vascular walls—the initial steps in leukocyte recruitment that is a hallmark of inflammation. Using the mOBM system, we studied the leukocyte-endothelial interactions in healthy and locally inflamed tissue and observed drastic changes in leukocyte movement (velocity and displacement profile). Our findings suggest that real-time imaging of leukocyte dynamics can provide new diagnostic insights (assessment of inflammation, temporal progression of disease, evaluation of therapeutic response, etc.) that are not available using conventional static parameters such as cell number and morphology.https://doi.org/10.1038/s41598-023-45018-1 |
| spellingShingle | Arutyun Bagramyan Charles P. Lin Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation Scientific Reports |
| title | Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation |
| title_full | Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation |
| title_fullStr | Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation |
| title_full_unstemmed | Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation |
| title_short | Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation |
| title_sort | miniaturized microscope for non invasive imaging of leukocyte endothelial interaction in human microcirculation |
| url | https://doi.org/10.1038/s41598-023-45018-1 |
| work_keys_str_mv | AT arutyunbagramyan miniaturizedmicroscopefornoninvasiveimagingofleukocyteendothelialinteractioninhumanmicrocirculation AT charlesplin miniaturizedmicroscopefornoninvasiveimagingofleukocyteendothelialinteractioninhumanmicrocirculation |