Utility of Bioluminescent Homogeneous Nucleotide Detection Assays in Measuring Activities of Nucleotide-Sugar Dependent Glycosyltransferases and Studying Their Inhibitors

Traditional glycosyltransferase (GT) activity assays are not easily configured for rapid detection nor for high throughput screening because they rely on radioactive product isolation, the use of heterogeneous immunoassays or mass spectrometry. In a typical glycosyltransferase biochemical reaction,...

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Bibliographic Details
Main Authors: Laurie Engel, Juliano Alves, Jacquelyn Hennek, Said A. Goueli, Hicham Zegzouti
Format: Article
Language:English
Published: MDPI AG 2021-10-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/26/20/6230