Development of a real-time loop-mediated isothermal amplification method for monitoring Pseudomonas lurida in raw milk throughout the year of pasture

Development of a real-time loop-mediated isothermal amplification method for monitoring Pseudomonas lurida in raw milk throughout the year of pasture

IntroductionThe psychrophilic bacterium Pseudomonas lurida (P. lurida) and its thermostable alkaline proteases can seriously damage raw milk quality.MethodsIn this study, specific primers were designed for P. lurida’s gyrB and aprX genes, and a real-time loop-mediated isothermal amplification (RealA...

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Bibliographic Details
Main Authors: Shufei Zhang, Lianxia Hu, Yuling Xue, Dong Zhang, Yaoguang Zhang, Shijie Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-04-01
Series:Frontiers in Microbiology
Subjects:
gyrB gene
aprX gene
real-time LAMP
Pseudomonas lurida
thermostable alkaline proteases
raw milk
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2023.1133077/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2023.1133077/full

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