Study of key amino acid residues of GH66 dextranase for producing high-degree polymerized isomaltooligosaccharides and improving of thermostability

Study of key amino acid residues of GH66 dextranase for producing high-degree polymerized isomaltooligosaccharides and improving of thermostability

Obtaining high-degree polymerized isomaltose is more difficult while achieving better prebiotic effects. We investigated the mutation specificity and enzymatic properties of SP5-Badex, a dextranase from the GH66 family of Bacillus aquimaris SP5, and determined its mutation sites through molecular do...

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Bibliographic Details
Main Authors: Qianru Lin, Huanyu Wang, Yingying Xu, Dongxue Dong, Qingzhen Miao, Jing Lu, Mingsheng Lyu, Shujun Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-08-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
dextranase
site-directed mutation
high-degree polymerization
isomaltooligosaccharide
molecular docking
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2022.961776/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2022.961776/full

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