Biogenic amine production by the wine <it>Lactobacillus brevis</it> IOEB 9809 in systems that partially mimic the gastrointestinal tract stress
<p>Abstract</p> <p>Background</p> <p>Ingestion of fermented foods containing high levels of biogenic amines (BA) can be deleterious to human health. Less obvious is the threat posed by BA producing organisms contained within the food which, in principle, could form BA a...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-10-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1471-2180/12/247 |
| _version_ | 1819117393835720704 |
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| author | Russo Pasquale Fernández de Palencia Pilar Romano Andrea Fernández María Lucas Patrick Spano Giuseppe López Paloma |
| author_facet | Russo Pasquale Fernández de Palencia Pilar Romano Andrea Fernández María Lucas Patrick Spano Giuseppe López Paloma |
| author_sort | Russo Pasquale |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Ingestion of fermented foods containing high levels of biogenic amines (BA) can be deleterious to human health. Less obvious is the threat posed by BA producing organisms contained within the food which, in principle, could form BA after ingestion even if the food product itself does not initially contain high BA levels. In this work we have investigated the production of tyramine and putrescine by <it>Lactobacillus brevis</it> IOEB 9809, of wine origin, under simulated gastrointestinal tract (GIT) conditions.</p> <p>Results</p> <p>An <it>in vitro</it> model that simulates the normal physiological conditions in the human digestive tract, as well as Caco-2 epithelial human cell lines, was used to challenge <it>L. brevis</it> IOEB 9809, which produced both tyramine and putrescine under all conditions tested. In the presence of BA precursors and under mild gastric stress, a correlation between enhancement of bacterial survival and a synchronous transcriptional activation of the tyramine and putrescine biosynthetic pathways was detected. High levels of both BA were observed after exposure of the bacterium to Caco-2 cells.</p> <p>Conclusions</p> <p><it>L. brevis</it> IOEB 9809 can produce tyramine and putrescine under simulated human digestive tract conditions. The results indicate that BA production may be a mechanism that increases bacterial survival under gastric stress.</p> |
| first_indexed | 2024-12-22T05:32:17Z |
| format | Article |
| id | doaj.art-ff3b340c6ea54095a4f602bdcd7cfe96 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-22T05:32:17Z |
| publishDate | 2012-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-ff3b340c6ea54095a4f602bdcd7cfe962022-12-21T18:37:25ZengBMCBMC Microbiology1471-21802012-10-0112124710.1186/1471-2180-12-247Biogenic amine production by the wine <it>Lactobacillus brevis</it> IOEB 9809 in systems that partially mimic the gastrointestinal tract stressRusso PasqualeFernández de Palencia PilarRomano AndreaFernández MaríaLucas PatrickSpano GiuseppeLópez Paloma<p>Abstract</p> <p>Background</p> <p>Ingestion of fermented foods containing high levels of biogenic amines (BA) can be deleterious to human health. Less obvious is the threat posed by BA producing organisms contained within the food which, in principle, could form BA after ingestion even if the food product itself does not initially contain high BA levels. In this work we have investigated the production of tyramine and putrescine by <it>Lactobacillus brevis</it> IOEB 9809, of wine origin, under simulated gastrointestinal tract (GIT) conditions.</p> <p>Results</p> <p>An <it>in vitro</it> model that simulates the normal physiological conditions in the human digestive tract, as well as Caco-2 epithelial human cell lines, was used to challenge <it>L. brevis</it> IOEB 9809, which produced both tyramine and putrescine under all conditions tested. In the presence of BA precursors and under mild gastric stress, a correlation between enhancement of bacterial survival and a synchronous transcriptional activation of the tyramine and putrescine biosynthetic pathways was detected. High levels of both BA were observed after exposure of the bacterium to Caco-2 cells.</p> <p>Conclusions</p> <p><it>L. brevis</it> IOEB 9809 can produce tyramine and putrescine under simulated human digestive tract conditions. The results indicate that BA production may be a mechanism that increases bacterial survival under gastric stress.</p>http://www.biomedcentral.com/1471-2180/12/247Biogenic aminesLactic acid bacteriaPutrescineTyramineFood safetyFood toxicity |
| spellingShingle | Russo Pasquale Fernández de Palencia Pilar Romano Andrea Fernández María Lucas Patrick Spano Giuseppe López Paloma Biogenic amine production by the wine <it>Lactobacillus brevis</it> IOEB 9809 in systems that partially mimic the gastrointestinal tract stress BMC Microbiology Biogenic amines Lactic acid bacteria Putrescine Tyramine Food safety Food toxicity |
| title | Biogenic amine production by the wine <it>Lactobacillus brevis</it> IOEB 9809 in systems that partially mimic the gastrointestinal tract stress |
| title_full | Biogenic amine production by the wine <it>Lactobacillus brevis</it> IOEB 9809 in systems that partially mimic the gastrointestinal tract stress |
| title_fullStr | Biogenic amine production by the wine <it>Lactobacillus brevis</it> IOEB 9809 in systems that partially mimic the gastrointestinal tract stress |
| title_full_unstemmed | Biogenic amine production by the wine <it>Lactobacillus brevis</it> IOEB 9809 in systems that partially mimic the gastrointestinal tract stress |
| title_short | Biogenic amine production by the wine <it>Lactobacillus brevis</it> IOEB 9809 in systems that partially mimic the gastrointestinal tract stress |
| title_sort | biogenic amine production by the wine it lactobacillus brevis it ioeb 9809 in systems that partially mimic the gastrointestinal tract stress |
| topic | Biogenic amines Lactic acid bacteria Putrescine Tyramine Food safety Food toxicity |
| url | http://www.biomedcentral.com/1471-2180/12/247 |
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