The sarcoplasmic protein profile of breast muscle in Turkeys in response to different dietary ratios of limiting amino acids and Clostridium perfringens-induced inflammation
ABSTRACT: In this study, the effects of the Arginine/Lysine (Arg/Lys) ratio in low- and high-methionine (Met) diets on the sarcoplasmic protein profile of breast muscles from turkeys reared under optimal or challenge (Clostridium perfringens infection) conditions were determined. One-day-old Hybrid...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122004849 |
| _version_ | 1811305121123926016 |
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| author | Paweł Konieczka Elżbieta Żelechowska Wiesław Przybylski Danuta Jaworska Piotr Sałek Misza Kinsner Jan Jankowski |
| author_facet | Paweł Konieczka Elżbieta Żelechowska Wiesław Przybylski Danuta Jaworska Piotr Sałek Misza Kinsner Jan Jankowski |
| author_sort | Paweł Konieczka |
| collection | DOAJ |
| description | ABSTRACT: In this study, the effects of the Arginine/Lysine (Arg/Lys) ratio in low- and high-methionine (Met) diets on the sarcoplasmic protein profile of breast muscles from turkeys reared under optimal or challenge (Clostridium perfringens infection) conditions were determined. One-day-old Hybrid Converter female turkey poults (216 in total) obtained from a commercial hatchery on hatching day, and on the basis of their average initial body weight were randomly allocated to 12 pens (4 m2 each; 2.0 m × 2.0 m) containing litter bedding and were reared over a 42-day experimental period. Diets with high levels of Lys contained approximately 1.80% and 1.65% Lys and were offered in two successive feeding periods (days 1–28 and days 29–42). The supplemental levels of Lys were consistent with the nutritional specifications for birds at their respective ages as established in the Management Guidelines for Raising Commercial Turkeys. The experiment was based on a completely randomized 3 × 2 × 2 factorial design with three levels of Arg (90%, 100% and 110%) relative to the content of dietary Met (30 or 45%) and without (−) or with (+) C. perfringens challenge at 34, 36, or 37 d of age. Meat samples were investigated in terms of pH, color, and sarcoplasmic protein profile. The experimental factors did not influence meat quality but the dietary Arg content affected meat color. The sarcoplasmic protein profile was influenced by all studied factors, and glycolytic enzymes were the most abundant. This study evidenced strong association between the challenge conditions and the involvement of glycolytic enzymes in cell metabolism, particularly in inflammatory processes, and DNA replication and maintenance in turkeys. The results showed an effect of C. perfringens infection and feeding with different doses of Arg and Met may lead to significant consequences in cell metabolism. |
| first_indexed | 2024-04-13T08:19:44Z |
| format | Article |
| id | doaj.art-ff3eb13682c54c6286672f73f359b713 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-13T08:19:44Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-ff3eb13682c54c6286672f73f359b7132022-12-22T02:54:41ZengElsevierPoultry Science0032-57912022-12-0110112102195The sarcoplasmic protein profile of breast muscle in Turkeys in response to different dietary ratios of limiting amino acids and Clostridium perfringens-induced inflammationPaweł Konieczka0Elżbieta Żelechowska1Wiesław Przybylski2Danuta Jaworska3Piotr Sałek4Misza Kinsner5Jan Jankowski6Department of Poultry Science and Apiculture, University of Warmia and Mazury, Olsztyn, Poland; Corresponding author:Institute of Human Nutrition Sciences, Warsaw University of Life Sciences, Warsaw, PolandInstitute of Human Nutrition Sciences, Warsaw University of Life Sciences, Warsaw, PolandInstitute of Human Nutrition Sciences, Warsaw University of Life Sciences, Warsaw, PolandInstitute of Human Nutrition Sciences, Warsaw University of Life Sciences, Warsaw, PolandDepartment of Animal Nutrition, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Jabłonna, PolandDepartment of Poultry Science and Apiculture, University of Warmia and Mazury, Olsztyn, PolandABSTRACT: In this study, the effects of the Arginine/Lysine (Arg/Lys) ratio in low- and high-methionine (Met) diets on the sarcoplasmic protein profile of breast muscles from turkeys reared under optimal or challenge (Clostridium perfringens infection) conditions were determined. One-day-old Hybrid Converter female turkey poults (216 in total) obtained from a commercial hatchery on hatching day, and on the basis of their average initial body weight were randomly allocated to 12 pens (4 m2 each; 2.0 m × 2.0 m) containing litter bedding and were reared over a 42-day experimental period. Diets with high levels of Lys contained approximately 1.80% and 1.65% Lys and were offered in two successive feeding periods (days 1–28 and days 29–42). The supplemental levels of Lys were consistent with the nutritional specifications for birds at their respective ages as established in the Management Guidelines for Raising Commercial Turkeys. The experiment was based on a completely randomized 3 × 2 × 2 factorial design with three levels of Arg (90%, 100% and 110%) relative to the content of dietary Met (30 or 45%) and without (−) or with (+) C. perfringens challenge at 34, 36, or 37 d of age. Meat samples were investigated in terms of pH, color, and sarcoplasmic protein profile. The experimental factors did not influence meat quality but the dietary Arg content affected meat color. The sarcoplasmic protein profile was influenced by all studied factors, and glycolytic enzymes were the most abundant. This study evidenced strong association between the challenge conditions and the involvement of glycolytic enzymes in cell metabolism, particularly in inflammatory processes, and DNA replication and maintenance in turkeys. The results showed an effect of C. perfringens infection and feeding with different doses of Arg and Met may lead to significant consequences in cell metabolism.http://www.sciencedirect.com/science/article/pii/S0032579122004849poultryamino acidsargininemethionineglycolytic enzymes |
| spellingShingle | Paweł Konieczka Elżbieta Żelechowska Wiesław Przybylski Danuta Jaworska Piotr Sałek Misza Kinsner Jan Jankowski The sarcoplasmic protein profile of breast muscle in Turkeys in response to different dietary ratios of limiting amino acids and Clostridium perfringens-induced inflammation Poultry Science poultry amino acids arginine methionine glycolytic enzymes |
| title | The sarcoplasmic protein profile of breast muscle in Turkeys in response to different dietary ratios of limiting amino acids and Clostridium perfringens-induced inflammation |
| title_full | The sarcoplasmic protein profile of breast muscle in Turkeys in response to different dietary ratios of limiting amino acids and Clostridium perfringens-induced inflammation |
| title_fullStr | The sarcoplasmic protein profile of breast muscle in Turkeys in response to different dietary ratios of limiting amino acids and Clostridium perfringens-induced inflammation |
| title_full_unstemmed | The sarcoplasmic protein profile of breast muscle in Turkeys in response to different dietary ratios of limiting amino acids and Clostridium perfringens-induced inflammation |
| title_short | The sarcoplasmic protein profile of breast muscle in Turkeys in response to different dietary ratios of limiting amino acids and Clostridium perfringens-induced inflammation |
| title_sort | sarcoplasmic protein profile of breast muscle in turkeys in response to different dietary ratios of limiting amino acids and clostridium perfringens induced inflammation |
| topic | poultry amino acids arginine methionine glycolytic enzymes |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122004849 |
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