A Cost-Effective Immobilization Method for MBP Fusion Proteins on Microtiter Plates Using a Gelatinized Starch–Agarose Mixture and Its Application for Convenient Protein–Protein Interaction Analysis

A Cost-Effective Immobilization Method for MBP Fusion Proteins on Microtiter Plates Using a Gelatinized Starch–Agarose Mixture and Its Application for Convenient Protein–Protein Interaction Analysis

The detection and quantification of protein–protein interactions (PPIs) is a crucial technique that often involves the use of recombinant proteins with fusion protein tags, such as maltose-binding protein (MBP) and glutathione-S-transferase (GST). In this study, we improved the cohesive and sticky p...

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Bibliographic Details
Main Authors: Yuri Emoto, Ryoya Katayama, Emi Hibino, Sho Ishihara, Natsuko Goda, Takeshi Tenno, Yoshihiro Kobashigawa, Hiroshi Morioka, Hidekazu Hiroaki
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Methods and Protocols
Subjects:
gelatinized starch
maltose-binding protein
microplate-based assay
protein–protein interaction
dissociation constant determination
Online Access:https://www.mdpi.com/2409-9279/6/3/44

Internet

https://www.mdpi.com/2409-9279/6/3/44

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