Simultaneous recording of multiple cellular signaling events by frequency- and spectrally-tuned multiplexing of fluorescent probes

Simultaneous recording of multiple cellular signaling events by frequency- and spectrally-tuned multiplexing of fluorescent probes

Fluorescent probes that change their spectral properties upon binding to small biomolecules, ions, or changes in the membrane potential (Vm) are invaluable tools to study cellular signaling pathways. Here, we introduce a novel technique for simultaneous recording of multiple probes at millisecond ti...

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Bibliographic Details
Main Authors: Michelina Kierzek, Parker E Deal, Evan W Miller, Shatanik Mukherjee, Dagmar Wachten, Arnd Baumann, U Benjamin Kaupp, Timo Strünker, Christoph Brenker
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-12-01
Series:eLife
Subjects:
Arbacia punctulata
fluorescence multiplexing
signal transduction
voltage-sensitive dye
Online Access:https://elifesciences.org/articles/63129

Internet

https://elifesciences.org/articles/63129

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