Multiplex Fluorescent RT-PCR to Quantify Leukemic Fusion Transcripts

The detection of chimeric transcripts derived from aberrant chromosomal fusion events provides an exceptionally valuable tool for the diagnosis of leukemia. We have developed a simple, inexpensive, reproducible, and automated method to quantify RT-PCR products. Our approach utilizes fluorescent PCR...

Full description

Bibliographic Details
Main Authors: M. Dupont, A. Goldsborough, T. Levayer, J. Savare, J.M. Rey, J.F. Rossi, J. Demaille, T. Lavabre-Bertrand
Format: Article
Language:English
Published: Taylor & Francis Group 2002-07-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/02331md04