A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice

A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice

Targeted CRISPR libraries expand the use of genetic screens across experimental conditions. Here, the authors develop a method for generating and analysing small scale custom CRISPR libraries and use it in the human and livestock pathogen Toxoplasma gondii to identify virulence factors in mice.

Detalhes bibliográficos
Principais autores: Joanna Young, Caia Dominicus, Jeanette Wagener, Simon Butterworth, Xingda Ye, Gavin Kelly, Merav Ordan, Becky Saunders, Rachael Instrell, Michael Howell, Aengus Stewart, Moritz Treeck
Formato: Artigo
Idioma:English
Publicado em: Nature Portfolio 2019-09-01
coleção:Nature Communications
Acesso em linha:https://doi.org/10.1038/s41467-019-11855-w

Internet

https://doi.org/10.1038/s41467-019-11855-w

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