Effects of Hydrogen Peroxide on In Vitro Cultures of Tea (<i>Camellia sinensis</i> L.) Grown in the Dark and in the Light: Morphology, Content of Malondialdehyde, and Accumulation of Various Polyphenols
Tea plants (<i>Camellia sinensis</i> L.) are phenol-accumulating crops that are widely used for public health. The healing effect of tea leaf products is due to the biosynthesis of such phenolic compounds (PCs) as flavans, which have P-vitamin capillary-strengthening activity. Due to the...
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MDPI AG
2022-10-01
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| author | Evgenia A. Goncharuk Maria Yu. Zubova Tatiana L. Nechaeva Varvara V. Kazantseva Alexander A. Gulevich Ekaterina N. Baranova Petr V. Lapshin Vera M. Katanskaya Maria A. Aksenova Natalia V. Zagoskina |
| author_facet | Evgenia A. Goncharuk Maria Yu. Zubova Tatiana L. Nechaeva Varvara V. Kazantseva Alexander A. Gulevich Ekaterina N. Baranova Petr V. Lapshin Vera M. Katanskaya Maria A. Aksenova Natalia V. Zagoskina |
| author_sort | Evgenia A. Goncharuk |
| collection | DOAJ |
| description | Tea plants (<i>Camellia sinensis</i> L.) are phenol-accumulating crops that are widely used for public health. The healing effect of tea leaf products is due to the biosynthesis of such phenolic compounds (PCs) as flavans, which have P-vitamin capillary-strengthening activity. Due to their limited habitat and the value of their specialized metabolites of a phenolic nature, a promising approach is to establish in vitro cultures from them that retain the ability to form PCs, which is characteristic of ex vivo tea plants. The aim of this study was to investigate the effect of exogenic H<sub>2</sub>O<sub>2</sub> (0.01 mM; 0.1 mM; 1 mM) on the growth, morphology, degree of stress response, and accumulation of various phenolic compounds in tea plant callus cultures of different ages (24 or 36 days) grown under different cultivation conditions (darkness or light). According to the results obtained, the H<sub>2</sub>O<sub>2</sub> effect on tea callus cultures of different ages did not cause changes in their morphophysiological characteristics, both after 2 h of exposure (rapid response of callus culture, RRCC) and after 48 h (delayed response of callus culture, DRCC). The determination of the malondialdehyde (MDA) content, which serves as an indicator of changes in the level of lipid peroxidation (LPO) and the presence of stress responses in plant cells, indicated either its maintenance at the control level, a decrease, or an increase. All these effects depended on the growth conditions of the tea callus cultures (darkness or light), their age, the duration of exposure (rapid or delayed response), and the H<sub>2</sub>O<sub>2</sub> concentration. Similar trends were noted for the total content of PCs as well as the amount of flavans, proanthocyanidins (soluble and insoluble forms), and lignin. The plant cell responses reflected changes in its adaptation programs, when specialized metabolites act as a target for the action of H<sub>2</sub>O<sub>2</sub>, thereby contributing to an increase in their resistance. |
| first_indexed | 2024-03-09T21:24:16Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:24:16Z |
| publishDate | 2022-10-01 |
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| series | Molecules |
| spelling | doaj.art-ff88ecbd4ae848058e0d81c0158236a52023-11-23T21:15:23ZengMDPI AGMolecules1420-30492022-10-012719667410.3390/molecules27196674Effects of Hydrogen Peroxide on In Vitro Cultures of Tea (<i>Camellia sinensis</i> L.) Grown in the Dark and in the Light: Morphology, Content of Malondialdehyde, and Accumulation of Various PolyphenolsEvgenia A. Goncharuk0Maria Yu. Zubova1Tatiana L. Nechaeva2Varvara V. Kazantseva3Alexander A. Gulevich4Ekaterina N. Baranova5Petr V. Lapshin6Vera M. Katanskaya7Maria A. Aksenova8Natalia V. Zagoskina9K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, RussiaAll-Russia Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, 127550 Moscow, RussiaAll-Russia Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, 127550 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, RussiaTea plants (<i>Camellia sinensis</i> L.) are phenol-accumulating crops that are widely used for public health. The healing effect of tea leaf products is due to the biosynthesis of such phenolic compounds (PCs) as flavans, which have P-vitamin capillary-strengthening activity. Due to their limited habitat and the value of their specialized metabolites of a phenolic nature, a promising approach is to establish in vitro cultures from them that retain the ability to form PCs, which is characteristic of ex vivo tea plants. The aim of this study was to investigate the effect of exogenic H<sub>2</sub>O<sub>2</sub> (0.01 mM; 0.1 mM; 1 mM) on the growth, morphology, degree of stress response, and accumulation of various phenolic compounds in tea plant callus cultures of different ages (24 or 36 days) grown under different cultivation conditions (darkness or light). According to the results obtained, the H<sub>2</sub>O<sub>2</sub> effect on tea callus cultures of different ages did not cause changes in their morphophysiological characteristics, both after 2 h of exposure (rapid response of callus culture, RRCC) and after 48 h (delayed response of callus culture, DRCC). The determination of the malondialdehyde (MDA) content, which serves as an indicator of changes in the level of lipid peroxidation (LPO) and the presence of stress responses in plant cells, indicated either its maintenance at the control level, a decrease, or an increase. All these effects depended on the growth conditions of the tea callus cultures (darkness or light), their age, the duration of exposure (rapid or delayed response), and the H<sub>2</sub>O<sub>2</sub> concentration. Similar trends were noted for the total content of PCs as well as the amount of flavans, proanthocyanidins (soluble and insoluble forms), and lignin. The plant cell responses reflected changes in its adaptation programs, when specialized metabolites act as a target for the action of H<sub>2</sub>O<sub>2</sub>, thereby contributing to an increase in their resistance.https://www.mdpi.com/1420-3049/27/19/6674hydrogen peroxidepolyphenolsin vitro<i>Camellia sinensis</i> L. |
| spellingShingle | Evgenia A. Goncharuk Maria Yu. Zubova Tatiana L. Nechaeva Varvara V. Kazantseva Alexander A. Gulevich Ekaterina N. Baranova Petr V. Lapshin Vera M. Katanskaya Maria A. Aksenova Natalia V. Zagoskina Effects of Hydrogen Peroxide on In Vitro Cultures of Tea (<i>Camellia sinensis</i> L.) Grown in the Dark and in the Light: Morphology, Content of Malondialdehyde, and Accumulation of Various Polyphenols Molecules hydrogen peroxide polyphenols in vitro <i>Camellia sinensis</i> L. |
| title | Effects of Hydrogen Peroxide on In Vitro Cultures of Tea (<i>Camellia sinensis</i> L.) Grown in the Dark and in the Light: Morphology, Content of Malondialdehyde, and Accumulation of Various Polyphenols |
| title_full | Effects of Hydrogen Peroxide on In Vitro Cultures of Tea (<i>Camellia sinensis</i> L.) Grown in the Dark and in the Light: Morphology, Content of Malondialdehyde, and Accumulation of Various Polyphenols |
| title_fullStr | Effects of Hydrogen Peroxide on In Vitro Cultures of Tea (<i>Camellia sinensis</i> L.) Grown in the Dark and in the Light: Morphology, Content of Malondialdehyde, and Accumulation of Various Polyphenols |
| title_full_unstemmed | Effects of Hydrogen Peroxide on In Vitro Cultures of Tea (<i>Camellia sinensis</i> L.) Grown in the Dark and in the Light: Morphology, Content of Malondialdehyde, and Accumulation of Various Polyphenols |
| title_short | Effects of Hydrogen Peroxide on In Vitro Cultures of Tea (<i>Camellia sinensis</i> L.) Grown in the Dark and in the Light: Morphology, Content of Malondialdehyde, and Accumulation of Various Polyphenols |
| title_sort | effects of hydrogen peroxide on in vitro cultures of tea i camellia sinensis i l grown in the dark and in the light morphology content of malondialdehyde and accumulation of various polyphenols |
| topic | hydrogen peroxide polyphenols in vitro <i>Camellia sinensis</i> L. |
| url | https://www.mdpi.com/1420-3049/27/19/6674 |
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