Achieving Optimal Transfection Conditions in Chicken Primordial Germ Cells Under Feeder- and Serum-Free Medium

Achieving Optimal Transfection Conditions in Chicken Primordial Germ Cells Under Feeder- and Serum-Free Medium

The successful application of primordial germ cells (PGCs) is an ideal method for generating gene-edited birds. However, barriers to efficient DNA transfection in PGCs lead to low transfection efficiency, limiting the generation of genetically modified chickens. The current study utilized chemical t...

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Bibliographic Details
Main Authors: Zhifeng Zhao, Xian Zou, Ying Zhu, Yanhua He, Endashaw Jebessa, Jiannan Zhang, Jian Ji, Peng Chen, Chenglong Luo
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Animals
Subjects:
primordial germ cell
feeder free
electroporation
transfection efficiency
CRISPR/Cas 9
Online Access:https://www.mdpi.com/2076-2615/15/4/590

Internet

https://www.mdpi.com/2076-2615/15/4/590

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