Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli

Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli

Abstract Background Co-expression of two distinct guide RNAs (gRNAs) has been used to facilitate the application of CRISPR/Cas9 system in fields such as large genomic deletion. The paired gRNAs are often placed adjacently in the same direction and expressed individually by two identical promoters, c...

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Bibliographic Details
Main Authors: Tingting Ding, Chaoyong Huang, Zeyu Liang, Xiaoyan Ma, Ning Wang, Yi-Xin Huo
Format: Article
Language:English
Published: BMC 2020-03-01
Series:Microbial Cell Factories
Subjects:
Plasmid stability
Paired gRNAs
Direct repeats
Inverted repeats
Large genomic deletion
Online Access:http://link.springer.com/article/10.1186/s12934-020-01321-4

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http://link.springer.com/article/10.1186/s12934-020-01321-4

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