Establishment of a serum-free culture method for human amnion mesenchymal stem cells
Objective To establish a method of serum free culture for human amnion mesenthymal stem cells. Methods The hAMSCs were isolated by trypsin-collagenase two step digestion method, then cultured in serum free medium. Then immunofluorescence and WST assay were used to detect cell phenotype and cells...
Main Author: | |
---|---|
Format: | Article |
Language: | zho |
Published: |
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-08-01
|
Series: | Jichu yixue yu linchuang |
Subjects: | |
Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200995.pdf |