Establishment of a serum-free culture method for human amnion mesenchymal stem cells

Objective To establish a method of serum free culture for human amnion mesenthymal stem cells. Methods The hAMSCs were isolated by trypsin-collagenase two step digestion method, then cultured in serum free medium. Then immunofluorescence and WST assay were used to detect cell phenotype and cells...

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Bibliographic Details
Main Author: LIU Xiao-yu, SHI Ping, PAN Bo-chen, PANG Xi-ning
Format: Article
Language:zho
Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. 2021-08-01
Series:Jichu yixue yu linchuang
Subjects:
Online Access:http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200995.pdf