Establishment of a serum-free culture method for human amnion mesenchymal stem cells
Objective To establish a method of serum free culture for human amnion mesenthymal stem cells. Methods The hAMSCs were isolated by trypsin-collagenase two step digestion method, then cultured in serum free medium. Then immunofluorescence and WST assay were used to detect cell phenotype and cells...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-08-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200995.pdf |
| _version_ | 1827390562459713536 |
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| author | LIU Xiao-yu, SHI Ping, PAN Bo-chen, PANG Xi-ning |
| author_facet | LIU Xiao-yu, SHI Ping, PAN Bo-chen, PANG Xi-ning |
| author_sort | LIU Xiao-yu, SHI Ping, PAN Bo-chen, PANG Xi-ning |
| collection | DOAJ |
| description | Objective To establish a method of serum free culture for human amnion mesenthymal stem cells. Methods The hAMSCs were isolated by trypsin-collagenase two step digestion method, then cultured in serum free medium. Then immunofluorescence and WST assay were used to detect cell phenotype and cells growth. Cell migration of hAMSCs cultured in serum free medium was examined by scratch wound closure assay. The expression of growth factor was detected by real time PCR assay. Results The hAMSCs cultured in serum free medium exhibited spindle-shape characteristics, were positive for CD73, CD90, but negative for CD45 and CD34. The cell proliferation showed a U-shaped curve and the average doubling time of was 72 hours.The scratch wound closure assay demonstrated a statistically significant increase of migration as compared with those cultured in FBS.The expression of VEGF,KGF,PDGF,IGF-1 was up-regulated compared with those cultured in FBS. Conclusions A serum free culture method of hAMSCs is established, which provides an experimental evidence supporting potential application. |
| first_indexed | 2024-03-08T16:54:31Z |
| format | Article |
| id | doaj.art-fff0f2f300304324b4553316a9ea783a |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:31Z |
| publishDate | 2021-08-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-fff0f2f300304324b4553316a9ea783a2024-01-05T03:06:25ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-08-0141811811185Establishment of a serum-free culture method for human amnion mesenchymal stem cellsLIU Xiao-yu, SHI Ping, PAN Bo-chen, PANG Xi-ning01. Center of Reproductive Medicine,Shengjing Hospital of China Medical University,Shenyang 110004;;2. Department of Stem Cells and Regenerative Medicine,China Medical University,Shenyang 110122;;3. Shenyang Amnion Bioengineering and Technology Research and Development Center Co.Ltd,Shenyang 110015,ChinaObjective To establish a method of serum free culture for human amnion mesenthymal stem cells. Methods The hAMSCs were isolated by trypsin-collagenase two step digestion method, then cultured in serum free medium. Then immunofluorescence and WST assay were used to detect cell phenotype and cells growth. Cell migration of hAMSCs cultured in serum free medium was examined by scratch wound closure assay. The expression of growth factor was detected by real time PCR assay. Results The hAMSCs cultured in serum free medium exhibited spindle-shape characteristics, were positive for CD73, CD90, but negative for CD45 and CD34. The cell proliferation showed a U-shaped curve and the average doubling time of was 72 hours.The scratch wound closure assay demonstrated a statistically significant increase of migration as compared with those cultured in FBS.The expression of VEGF,KGF,PDGF,IGF-1 was up-regulated compared with those cultured in FBS. Conclusions A serum free culture method of hAMSCs is established, which provides an experimental evidence supporting potential application.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200995.pdfhuman amnion mesenchymal stem cells (hamscs)|serum free culture|proliferation|migration |
| spellingShingle | LIU Xiao-yu, SHI Ping, PAN Bo-chen, PANG Xi-ning Establishment of a serum-free culture method for human amnion mesenchymal stem cells Jichu yixue yu linchuang human amnion mesenchymal stem cells (hamscs)|serum free culture|proliferation|migration |
| title | Establishment of a serum-free culture method for human amnion mesenchymal stem cells |
| title_full | Establishment of a serum-free culture method for human amnion mesenchymal stem cells |
| title_fullStr | Establishment of a serum-free culture method for human amnion mesenchymal stem cells |
| title_full_unstemmed | Establishment of a serum-free culture method for human amnion mesenchymal stem cells |
| title_short | Establishment of a serum-free culture method for human amnion mesenchymal stem cells |
| title_sort | establishment of a serum free culture method for human amnion mesenchymal stem cells |
| topic | human amnion mesenchymal stem cells (hamscs)|serum free culture|proliferation|migration |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200995.pdf |
| work_keys_str_mv | AT liuxiaoyushipingpanbochenpangxining establishmentofaserumfreeculturemethodforhumanamnionmesenchymalstemcells |