Cloning-free CRISPR

Cloning-free CRISPR

We present self-cloning CRISPR/Cas9 (scCRISPR), a technology that allows for CRISPR/Cas9-mediated genomic mutation and site-specific knockin transgene creation within several hours by circumventing the need to clone a site-specific single-guide RNA (sgRNA) or knockin homology construct for each targ...

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Bibliographic Details
Main Authors: Arbab, Mandana, Srinivasan, Sharanya, Geijsen, Niels, Sherwood, Richard I., Hashimoto, Tatsunori Benjamin
Other Authors: Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Format: Article
Language:en_US
Published: Elsevier 2016
Online Access:http://hdl.handle.net/1721.1/100809
https://orcid.org/0000-0003-0521-5855

Internet

http://hdl.handle.net/1721.1/100809
https://orcid.org/0000-0003-0521-5855

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