Cloning-free CRISPR
We present self-cloning CRISPR/Cas9 (scCRISPR), a technology that allows for CRISPR/Cas9-mediated genomic mutation and site-specific knockin transgene creation within several hours by circumventing the need to clone a site-specific single-guide RNA (sgRNA) or knockin homology construct for each targ...
| Main Authors: | , , , , |
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| 其他作者: | |
| 格式: | 文件 |
| 语言: | en_US |
| 出版: |
Elsevier
2016
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| 在线阅读: | http://hdl.handle.net/1721.1/100809 https://orcid.org/0000-0003-0521-5855 |