A transcription blocker isolated from a designed repeat protein combinatorial library by in vivo functional screen

A transcription blocker isolated from a designed repeat protein combinatorial library by in vivo functional screen

A highly diverse DNA library coding for ankyrin seven-repeat proteins (ANK-N5C) was designed and constructed by a PCR-based combinatorial assembly strategy. A bacterial melibiose fermentation assay was adapted for in vivo functional screen. We isolated a transcription blocker that completely inhibit...

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Bibliographic Details
Main Authors: Tikhonova, Elena B., Ethayathulla, Abdul S., Su, Yue, Hariharan, Parameswaran, Xie, Shicong, Guan, Lan
Other Authors: Massachusetts Institute of Technology. Computational and Systems Biology Program
Format: Article
Language:en_US
Published: Nature Publishing Group 2016
Online Access:http://hdl.handle.net/1721.1/101892
https://orcid.org/0000-0002-3283-3248

Internet

http://hdl.handle.net/1721.1/101892
https://orcid.org/0000-0002-3283-3248

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