Optogenetic drive of neocortical pyramidal neurons generates fMRI signals that are correlated with spiking activity
Local fluctuations in the blood oxygenation level-dependent (BOLD) signal serve as the basis of functional magnetic resonance imaging (fMRI). Understanding the correlation between distinct aspects of neural activity and the BOLD response is fundamental to the interpretation of this widely used mappi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2016
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| Online Access: | http://hdl.handle.net/1721.1/102221 https://orcid.org/0000-0002-7472-5480 https://orcid.org/0000-0002-0419-3351 https://orcid.org/0000-0002-8381-7555 https://orcid.org/0000-0002-0756-5587 https://orcid.org/0000-0002-4326-7720 |
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| author | Kahn, I. Knoblich, Ulf Desai, Mitul Bernstein, Jacob G. Moore, Christopher I. Buckner, R. L. Graybiel, Ann M Boyden, Edward |
| author2 | Massachusetts Institute of Technology. Synthetic Neurobiology Group |
| author_facet | Massachusetts Institute of Technology. Synthetic Neurobiology Group Kahn, I. Knoblich, Ulf Desai, Mitul Bernstein, Jacob G. Moore, Christopher I. Buckner, R. L. Graybiel, Ann M Boyden, Edward |
| author_sort | Kahn, I. |
| collection | MIT |
| description | Local fluctuations in the blood oxygenation level-dependent (BOLD) signal serve as the basis of functional magnetic resonance imaging (fMRI). Understanding the correlation between distinct aspects of neural activity and the BOLD response is fundamental to the interpretation of this widely used mapping signal. Analysis of this question requires the ability to precisely manipulate the activity of defined neurons. To achieve such control, we combined optogenetic drive of neocortical neurons with high-resolution (9.4 T) rodent fMRI and detailed analysis of neurophysiological data. Light-driven activation of pyramidal neurons resulted in a positive BOLD response at the stimulated site. To help differentiate the neurophysiological correlate(s) of the BOLD response, we employed light trains of the same average frequency, but with periodic and Poisson distributed pulse times. These different types of pulse trains generated dissociable patterns of single-unit, multi-unit and local field potential (LFP) activity, and of BOLD signals. The BOLD activity exhibited the strongest correlation to spiking activity with increasing rates of stimulation, and, to a first approximation, was linear with pulse delivery rate, while LFP activity showed a weaker correlation. These data provide an example of a strong correlation between spike rate and the BOLD response. |
| first_indexed | 2024-09-23T12:44:19Z |
| format | Article |
| id | mit-1721.1/102221 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:44:19Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1022212022-10-01T10:50:18Z Optogenetic drive of neocortical pyramidal neurons generates fMRI signals that are correlated with spiking activity Kahn, I. Knoblich, Ulf Desai, Mitul Bernstein, Jacob G. Moore, Christopher I. Buckner, R. L. Graybiel, Ann M Boyden, Edward Massachusetts Institute of Technology. Synthetic Neurobiology Group Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Media Laboratory McGovern Institute for Brain Research at MIT Program in Media Arts and Sciences (Massachusetts Institute of Technology) Knoblich, Ulf Desai, Mitul Bernstein, Jacob G. Graybiel, Ann M. Boyden, Edward Stuart Moore, Christopher I. Local fluctuations in the blood oxygenation level-dependent (BOLD) signal serve as the basis of functional magnetic resonance imaging (fMRI). Understanding the correlation between distinct aspects of neural activity and the BOLD response is fundamental to the interpretation of this widely used mapping signal. Analysis of this question requires the ability to precisely manipulate the activity of defined neurons. To achieve such control, we combined optogenetic drive of neocortical neurons with high-resolution (9.4 T) rodent fMRI and detailed analysis of neurophysiological data. Light-driven activation of pyramidal neurons resulted in a positive BOLD response at the stimulated site. To help differentiate the neurophysiological correlate(s) of the BOLD response, we employed light trains of the same average frequency, but with periodic and Poisson distributed pulse times. These different types of pulse trains generated dissociable patterns of single-unit, multi-unit and local field potential (LFP) activity, and of BOLD signals. The BOLD activity exhibited the strongest correlation to spiking activity with increasing rates of stimulation, and, to a first approximation, was linear with pulse delivery rate, while LFP activity showed a weaker correlation. These data provide an example of a strong correlation between spike rate and the BOLD response. National Science Foundation (U.S.) National Institutes of Health (U.S.) Howard Hughes Medical Institute McGovern Institute for Brain Research at MIT 2016-04-08T16:27:44Z 2016-04-08T16:27:44Z 2013-05 Article http://purl.org/eprint/type/JournalArticle 00068993 http://hdl.handle.net/1721.1/102221 Kahn, I., U. Knoblich, M. Desai, J. Bernstein, A.M. Graybiel, E.S. Boyden, R.L. Buckner, and C.I. Moore. “Optogenetic Drive of Neocortical Pyramidal Neurons Generates fMRI Signals That Are Correlated with Spiking Activity.” Brain Research 1511 (May 2013): 33–45. https://orcid.org/0000-0002-7472-5480 https://orcid.org/0000-0002-0419-3351 https://orcid.org/0000-0002-8381-7555 https://orcid.org/0000-0002-0756-5587 https://orcid.org/0000-0002-4326-7720 en_US http://dx.doi.org/10.1016/j.brainres.2013.03.011 Brain Research Creative Commons Attribution-Noncommercial-NoDerivatives http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Kahn, I. Knoblich, Ulf Desai, Mitul Bernstein, Jacob G. Moore, Christopher I. Buckner, R. L. Graybiel, Ann M Boyden, Edward Optogenetic drive of neocortical pyramidal neurons generates fMRI signals that are correlated with spiking activity |
| title | Optogenetic drive of neocortical pyramidal neurons generates fMRI signals that are correlated with spiking activity |
| title_full | Optogenetic drive of neocortical pyramidal neurons generates fMRI signals that are correlated with spiking activity |
| title_fullStr | Optogenetic drive of neocortical pyramidal neurons generates fMRI signals that are correlated with spiking activity |
| title_full_unstemmed | Optogenetic drive of neocortical pyramidal neurons generates fMRI signals that are correlated with spiking activity |
| title_short | Optogenetic drive of neocortical pyramidal neurons generates fMRI signals that are correlated with spiking activity |
| title_sort | optogenetic drive of neocortical pyramidal neurons generates fmri signals that are correlated with spiking activity |
| url | http://hdl.handle.net/1721.1/102221 https://orcid.org/0000-0002-7472-5480 https://orcid.org/0000-0002-0419-3351 https://orcid.org/0000-0002-8381-7555 https://orcid.org/0000-0002-0756-5587 https://orcid.org/0000-0002-4326-7720 |
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