Rationally engineered Cas9 nucleases with improved specificity

Rationally engineered Cas9 nucleases with improved specificity

The RNA-guided endonuclease Cas9 is a versatile genome-editing tool with a broad range of applications from therapeutics to functional annotation of genes. Cas9 creates double-strand breaks (DSBs) at targeted genomic loci complementary to a short RNA guide. However, Cas9 can cleave off-target sites...

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Bibliographic Details
Main Authors: Slaymaker, Ian, Gao, Linyi, Zetsche, Bernd, Scott, David Arthur, Yan, Winston Xia, Zhang, Feng
Other Authors: Harvard University--MIT Division of Health Sciences and Technology
Format: Article
Language:en_US
Published: American Association for the Advancement of Science (AAAS) 2016
Online Access:http://hdl.handle.net/1721.1/102582
https://orcid.org/0000-0003-2782-2509
https://orcid.org/0000-0002-3067-479X
https://orcid.org/0000-0002-2639-9879
https://orcid.org/0000-0002-3579-0327
https://orcid.org/0000-0001-8794-2137

Internet

http://hdl.handle.net/1721.1/102582
https://orcid.org/0000-0003-2782-2509
https://orcid.org/0000-0002-3067-479X
https://orcid.org/0000-0002-2639-9879
https://orcid.org/0000-0002-3579-0327
https://orcid.org/0000-0001-8794-2137

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