Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease

Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease

We have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. These sgRNAs, with 14- to 15-bp target sequences and MS2 binding loops, can activate gene expression using an active St...

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Bibliographic Details
Main Authors: Joung, Julia, Zhang, Feng, Konermann, Silvana, Dahlman, James E., Abudayyeh, Omar Osama, Gootenberg, Jonathan S.
Other Authors: Harvard University--MIT Division of Health Sciences and Technology
Format: Article
Language:en_US
Published: Nature Publishing Group 2016
Online Access:http://hdl.handle.net/1721.1/102583
https://orcid.org/0000-0003-2782-2509
https://orcid.org/0000-0001-7915-1685
https://orcid.org/0000-0001-6656-5002
https://orcid.org/0000-0002-7979-3220

Internet

http://hdl.handle.net/1721.1/102583
https://orcid.org/0000-0003-2782-2509
https://orcid.org/0000-0001-7915-1685
https://orcid.org/0000-0001-6656-5002
https://orcid.org/0000-0002-7979-3220

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