Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

Systematic interrogation of gene function requires the ability to perturb gene expression in a robust and generalizable manner. Here we describe structure-guided engineering of a CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous genomic loci. We used these engineered...

Full description

Bibliographic Details
Main Authors: Konermann, Silvana, Brigham, Mark D., Trevino, Alexandro E., Joung, Julia, Barcena, Clea, Hsu, Patrick D., Habib, Naomi, Gootenberg, Jonathan S., Nishimasu, Hiroshi, Nureki, Osamu, Zhang, Feng, Abudayyeh, Omar Osama
Other Authors: Harvard University--MIT Division of Health Sciences and Technology
Format: Article
Language:en_US
Published: 2016
Online Access:http://hdl.handle.net/1721.1/102589
https://orcid.org/0000-0003-2782-2509
https://orcid.org/0000-0001-7915-1685
https://orcid.org/0000-0001-6656-5002
https://orcid.org/0000-0002-7979-3220

Internet

http://hdl.handle.net/1721.1/102589
https://orcid.org/0000-0003-2782-2509
https://orcid.org/0000-0001-7915-1685
https://orcid.org/0000-0001-6656-5002
https://orcid.org/0000-0002-7979-3220

Similar Items