Cas9 Functionally Opens Chromatin
Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at prev...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Public Library of Science
2016
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| Online Access: | http://hdl.handle.net/1721.1/102604 https://orcid.org/0000-0003-0521-5855 https://orcid.org/0000-0003-1709-4034 |
| _version_ | 1826213820861775872 |
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| author | Barkal, Amira A. Srinivasan, Sharanya Gifford, David K. Sherwood, Richard I. Hashimoto, Tatsunori Benjamin |
| author2 | Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory |
| author_facet | Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory Barkal, Amira A. Srinivasan, Sharanya Gifford, David K. Sherwood, Richard I. Hashimoto, Tatsunori Benjamin |
| author_sort | Barkal, Amira A. |
| collection | MIT |
| description | Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding. |
| first_indexed | 2024-09-23T15:55:21Z |
| format | Article |
| id | mit-1721.1/102604 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:55:21Z |
| publishDate | 2016 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/1026042022-10-02T05:05:25Z Cas9 Functionally Opens Chromatin Barkal, Amira A. Srinivasan, Sharanya Gifford, David K. Sherwood, Richard I. Hashimoto, Tatsunori Benjamin Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Barkal, Amira A. Hashimoto, Tatsunori Benjamin Gifford, David K. Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding. National Institutes of Health (U.S.) (5UL1DE019581) National Institutes of Health (U.S.) (RL1DE019021) National Institutes of Health (U.S.) (1K01DK101684-01) National Institutes of Health (U.S.) (1U01HG007037) National Institutes of Health (U.S.) (5P01NS055923) 2016-05-23T14:44:15Z 2016-05-23T14:44:15Z 2016-03 2015-10 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/102604 Barkal, Amira A., Sharanya Srinivasan, Tatsunori Hashimoto, David K. Gifford, and Richard I. Sherwood. “Cas9 Functionally Opens Chromatin.” Edited by Hodaka Fujii. PLOS ONE 11, no. 3 (March 31, 2016): e0152683. https://orcid.org/0000-0003-0521-5855 https://orcid.org/0000-0003-1709-4034 en_US http://dx.doi.org/10.1371/journal.pone.0152683 PLOS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science Public Library of Science |
| spellingShingle | Barkal, Amira A. Srinivasan, Sharanya Gifford, David K. Sherwood, Richard I. Hashimoto, Tatsunori Benjamin Cas9 Functionally Opens Chromatin |
| title | Cas9 Functionally Opens Chromatin |
| title_full | Cas9 Functionally Opens Chromatin |
| title_fullStr | Cas9 Functionally Opens Chromatin |
| title_full_unstemmed | Cas9 Functionally Opens Chromatin |
| title_short | Cas9 Functionally Opens Chromatin |
| title_sort | cas9 functionally opens chromatin |
| url | http://hdl.handle.net/1721.1/102604 https://orcid.org/0000-0003-0521-5855 https://orcid.org/0000-0003-1709-4034 |
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