DNA targeting specificity of RNA-guided Cas9 nucleases

DNA targeting specificity of RNA-guided Cas9 nucleases

The Streptococcus pyogenes Cas9 (SpCas9) nuclease can be efficiently targeted to genomic loci by means of single-guide RNAs (sgRNAs) to enable genome editing1, 2, 3, 4, 5, 6, 7, 8, 9, 10. Here, we characterize SpCas9 targeting specificity in human cells to inform the selection of target sites and av...

Full description

Bibliographic Details
Main Authors: Hsu, Patrick, Scott, David Arthur, Weinstein, Joshua, Ran, F. Ann, Konermann, Silvana M., Agarwala, Vineeta, Li, Yinqing, Fine, Eli J., Wu, Xavier, Shalem, Ophir, Cradick, Thomas J., Marraffini, Luciano A., Bao, Gang, Zhang, Feng
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:en_US
Published: Nature Publishing Group 2016
Online Access:http://hdl.handle.net/1721.1/102691
https://orcid.org/0000-0003-2782-2509
https://orcid.org/0000-0001-7915-1685
https://orcid.org/0000-0002-2639-9879

Internet

http://hdl.handle.net/1721.1/102691
https://orcid.org/0000-0003-2782-2509
https://orcid.org/0000-0001-7915-1685
https://orcid.org/0000-0002-2639-9879

Similar Items