RNA-guided editing of bacterial genomes using CRISPR-Cas systems

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

Here we use the clustered, regularly interspaced, short palindromic repeats (CRISPR)–associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations in the genomes of Streptococcus pneumoniae and Escherichia coli. The approach relies on dual-RNA:Cas9-directed cleavage at the tar...

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Bibliographic Details
Main Authors: Jiang, Wenyan, Bikard, David, Cox, David Daniel, Zhang, Feng, Marraffini, Luciano A.
Other Authors: Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Format: Article
Language:en_US
Published: Nature Publishing Group 2016
Online Access:http://hdl.handle.net/1721.1/102692
https://orcid.org/0000-0003-2782-2509
https://orcid.org/0000-0002-2189-9743

Internet

http://hdl.handle.net/1721.1/102692
https://orcid.org/0000-0003-2782-2509
https://orcid.org/0000-0002-2189-9743

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