RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Here we use the clustered, regularly interspaced, short palindromic repeats (CRISPR)–associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations in the genomes of Streptococcus pneumoniae and Escherichia coli. The approach relies on dual-RNA:Cas9-directed cleavage at the tar...
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2016
|
| Online Access: | http://hdl.handle.net/1721.1/102692 https://orcid.org/0000-0003-2782-2509 https://orcid.org/0000-0002-2189-9743 |