Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

Targeted genome editing technologies have enabled a broad range of research and medical applications. The Cas9 nuclease from the microbial CRISPR-Cas system is targeted to specific genomic loci by a 20 nt guide sequence, which can tolerate certain mismatches to the DNA target and thereby promote und...

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Bibliographic Details
Main Authors: Ran, F. Ann, Hsu, Patrick D., Lin, Chie Yu, Gootenberg, Jonathan S., Konermann, Silvana M., Trevino, Alexandro E., Scott, David A., Inoue, Azusa, Matoba, Shogo, Zhang, Yi, Zhang, Feng, Ran, F. Ann, Scott, David Arthur, Hsu, Patrick D.
Other Authors: Institute for Medical Engineering and Science
Format: Article
Language:en_US
Published: Cell Press/Elsevier 2016
Online Access:http://hdl.handle.net/1721.1/102782
https://orcid.org/0000-0003-2782-2509
https://orcid.org/0000-0001-7915-1685
https://orcid.org/0000-0002-2639-9879

Internet

http://hdl.handle.net/1721.1/102782
https://orcid.org/0000-0003-2782-2509
https://orcid.org/0000-0001-7915-1685
https://orcid.org/0000-0002-2639-9879

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