Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2
This protocol describes a method to obtain spatially resolved proteomic maps of specific compartments within living mammalian cells. An engineered peroxidase, APEX2, is genetically targeted to a cellular region of interest. Upon the addition of hydrogen peroxide for 1 min to cells preloaded with a b...
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2016
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| Online Access: | http://hdl.handle.net/1721.1/104056 https://orcid.org/0000-0002-2687-3470 https://orcid.org/0000-0003-4994-2392 https://orcid.org/0000-0002-8277-5226 https://orcid.org/0000-0003-3972-2820 https://orcid.org/0000-0001-8156-1915 |