Viral Packaging and Cell Culture for CRISPR-Based Screens

This protocol describes how to perform the tissue culture and high-throughput sequencing library preparation for a CRISPR-based screen. First, pantropic lentivirus is prepared from a sgRNA plasmid pool and applied to the target cells. Following antibiotic selection and a harvest of the initial popul...

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Bibliographic Details
Main Authors: Wang, Tim, Lander, Eric Steven, Sabatini, David
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:en_US
Published: Cold Spring Harbor Laboratory Press 2016
Online Access:http://hdl.handle.net/1721.1/105364
https://orcid.org/0000-0002-4227-5163
https://orcid.org/0000-0002-1446-7256