Viral Packaging and Cell Culture for CRISPR-Based Screens

This protocol describes how to perform the tissue culture and high-throughput sequencing library preparation for a CRISPR-based screen. First, pantropic lentivirus is prepared from a sgRNA plasmid pool and applied to the target cells. Following antibiotic selection and a harvest of the initial popul...

Täydet tiedot

Bibliografiset tiedot
Päätekijät: Wang, Tim, Lander, Eric Steven, Sabatini, David
Muut tekijät: Massachusetts Institute of Technology. Department of Biology
Aineistotyyppi: Artikkeli
Kieli:en_US
Julkaistu: Cold Spring Harbor Laboratory Press 2016
Linkit:http://hdl.handle.net/1721.1/105364
https://orcid.org/0000-0002-4227-5163
https://orcid.org/0000-0002-1446-7256