The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones
Protein AMPylation by Fic domain-containing proteins (Fic proteins) is an ancient and conserved post-translational modification of mostly unexplored significance. Here we characterize the Caenorhabditis elegans Fic protein FIC-1 in vitro and in vivo. FIC-1 is an AMPylase that localizes to the nuclea...
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2016
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| Online Access: | http://hdl.handle.net/1721.1/105785 https://orcid.org/0000-0001-5338-0973 https://orcid.org/0000-0001-6819-8832 https://orcid.org/0000-0001-8012-1512 https://orcid.org/0000-0002-1090-6071 |
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| author | Truttmann, Matthias Cruz, Victor Emmanuel Guo, Xuanzong Engert, Christoph Schwartz, Thomas Ploegh, Hidde |
| author2 | Massachusetts Institute of Technology. Computational and Systems Biology Program |
| author_facet | Massachusetts Institute of Technology. Computational and Systems Biology Program Truttmann, Matthias Cruz, Victor Emmanuel Guo, Xuanzong Engert, Christoph Schwartz, Thomas Ploegh, Hidde |
| author_sort | Truttmann, Matthias |
| collection | MIT |
| description | Protein AMPylation by Fic domain-containing proteins (Fic proteins) is an ancient and conserved post-translational modification of mostly unexplored significance. Here we characterize the Caenorhabditis elegans Fic protein FIC-1 in vitro and in vivo. FIC-1 is an AMPylase that localizes to the nuclear surface and modifies core histones H2 and H3 as well as heat shock protein 70 family members and translation elongation factors. The three-dimensional structure of FIC-1 is similar to that of its human ortholog, HYPE, with 38% sequence identity. We identify a link between FIC-1-mediated AMPylation and susceptibility to the pathogen Pseudomonas aeruginosa, establishing a connection between AMPylation and innate immunity in C. elegans. |
| first_indexed | 2024-09-23T11:20:55Z |
| format | Article |
| id | mit-1721.1/105785 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:20:55Z |
| publishDate | 2016 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/1057852022-09-27T18:57:52Z The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones Truttmann, Matthias Cruz, Victor Emmanuel Guo, Xuanzong Engert, Christoph Schwartz, Thomas Ploegh, Hidde Massachusetts Institute of Technology. Computational and Systems Biology Program Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Truttmann, Matthias Cruz, Victor Emmanuel Guo, Xuanzong Engert, Christoph Schwartz, Thomas Ploegh, Hidde Protein AMPylation by Fic domain-containing proteins (Fic proteins) is an ancient and conserved post-translational modification of mostly unexplored significance. Here we characterize the Caenorhabditis elegans Fic protein FIC-1 in vitro and in vivo. FIC-1 is an AMPylase that localizes to the nuclear surface and modifies core histones H2 and H3 as well as heat shock protein 70 family members and translation elongation factors. The three-dimensional structure of FIC-1 is similar to that of its human ortholog, HYPE, with 38% sequence identity. We identify a link between FIC-1-mediated AMPylation and susceptibility to the pathogen Pseudomonas aeruginosa, establishing a connection between AMPylation and innate immunity in C. elegans. National Institutes of Health (U.S.) (P41 GM103403) Swiss National Science Foundation (Advanced Postdoc Mobility Fellowship) National Institutes of Health (U.S.) (NIH Pioneer Award (DP01)) 2016-12-09T19:51:39Z 2016-12-09T19:51:39Z 2016-05 2015-09 Article http://purl.org/eprint/type/JournalArticle 1553-7404 http://hdl.handle.net/1721.1/105785 Truttmann, Matthias C., Victor E. Cruz, Xuanzong Guo, Christoph Engert, Thomas U. Schwartz, and Hidde L. Ploegh. “The Caenorhabditis Elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones.” Edited by Man-Wah Tan. PLOS Genetics 12, no. 5 (May 3, 2016): e1006023. https://orcid.org/0000-0001-5338-0973 https://orcid.org/0000-0001-6819-8832 https://orcid.org/0000-0001-8012-1512 https://orcid.org/0000-0002-1090-6071 en_US http://dx.doi.org/10.1371/journal.pgen.1006023 PLOS Genetics Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science PLOS |
| spellingShingle | Truttmann, Matthias Cruz, Victor Emmanuel Guo, Xuanzong Engert, Christoph Schwartz, Thomas Ploegh, Hidde The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones |
| title | The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones |
| title_full | The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones |
| title_fullStr | The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones |
| title_full_unstemmed | The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones |
| title_short | The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones |
| title_sort | caenorhabditis elegans protein fic 1 is an ampylase that covalently modifies heat shock 70 family proteins translation elongation factors and histones |
| url | http://hdl.handle.net/1721.1/105785 https://orcid.org/0000-0001-5338-0973 https://orcid.org/0000-0001-6819-8832 https://orcid.org/0000-0001-8012-1512 https://orcid.org/0000-0002-1090-6071 |
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