Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction

Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction

We present a scalable, integrated strategy for coupled protein and RNA detection from single cells. Our approach leverages the DNA polymerase activity of reverse transcriptase to simultaneously perform proximity extension assays and complementary DNA synthesis in the same reaction. Using the Fluidig...

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Bibliographic Details
Main Authors: Li, Shuqiang, Darmanis, Spyros, Lundberg, Martin, Fredriksson, Simon, Landegren, Ulf, Gallant, Caroline J., Livak, Kenneth J., Genshaft, Alex S., Prakadan, Sanjay, Ziegler, Carly, Hong, Joyce, Regev, Aviv, Shalek, Alexander K
Other Authors: Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Format: Article
Language:English
Published: BioMed Central 2016
Online Access:http://hdl.handle.net/1721.1/105800
https://orcid.org/0000-0003-3079-5134
https://orcid.org/0000-0002-5621-8768
https://orcid.org/0000-0002-8279-7150
https://orcid.org/0000-0001-8567-2049

Internet

http://hdl.handle.net/1721.1/105800
https://orcid.org/0000-0003-3079-5134
https://orcid.org/0000-0002-5621-8768
https://orcid.org/0000-0002-8279-7150
https://orcid.org/0000-0001-8567-2049

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