Large-Scale Single Guide RNA Library Construction and Use for CRISPR–Cas9-Based Genetic Screens

The ability to systematically disrupt genes serves as a powerful tool for understanding their function. The programmable CRISPR–Cas9 system enables efficient targeting of large numbers of genes through the use of single guide RNA (sgRNA) libraries. In cultured mammalian cells, collections of knockou...

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Detaylı Bibliyografya
Asıl Yazarlar: Wang, Tim, Lander, Eric Steven, Sabatini, David
Diğer Yazarlar: Massachusetts Institute of Technology. Department of Biology
Materyal Türü: Makale
Dil:en_US
Baskı/Yayın Bilgisi: Cold Spring Harbor Laboratory Press 2016
Online Erişim:http://hdl.handle.net/1721.1/105931
https://orcid.org/0000-0002-4227-5163
https://orcid.org/0000-0002-1446-7256