Large-Scale Single Guide RNA Library Construction and Use for CRISPR–Cas9-Based Genetic Screens
The ability to systematically disrupt genes serves as a powerful tool for understanding their function. The programmable CRISPR–Cas9 system enables efficient targeting of large numbers of genes through the use of single guide RNA (sgRNA) libraries. In cultured mammalian cells, collections of knockou...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Cold Spring Harbor Laboratory Press
2016
|
| Online Access: | http://hdl.handle.net/1721.1/105931 https://orcid.org/0000-0002-4227-5163 https://orcid.org/0000-0002-1446-7256 |