Identification and characterization of essential genes in the human genome
Large-scale genetic analysis of lethal phenotypes has elucidated the molecular underpinnings of many biological processes. Using the bacterial clustered regularly interspaced short palindromic repeats (CRISPR) system, we constructed a genome-wide single-guide RNA (sgRNA) library to screen for genes...
Main Authors: | , , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | en_US |
Published: |
American Association for the Advancement of Science (AAAS)
2017
|
Online Access: | http://hdl.handle.net/1721.1/106204 https://orcid.org/0000-0002-4227-5163 https://orcid.org/0000-0002-1446-7256 |