Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

Methods to introduce targeted double-strand breaks (DSBs) into DNA enable precise genome editing by increasing the rate at which externally supplied DNA fragments are incorporated into the genome through homologous recombination. The efficiency of these methods is limited by nonhomologous end joinin...

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Bibliografiske detaljer
Main Authors: Maruyama, Takeshi, Dougan, Stephanie K, Truttmann, Matthias C, Bilate, Angelina M, Ingram, Jessica R, Ploegh, Hidde
Andre forfattere: Massachusetts Institute of Technology. Department of Biology
Format: Article
Sprog:en_US
Udgivet: Nature Publishing Group 2017
Online adgang:http://hdl.handle.net/1721.1/106223
https://orcid.org/0000-0002-1090-6071