Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

Methods to introduce targeted double-strand breaks (DSBs) into DNA enable precise genome editing by increasing the rate at which externally supplied DNA fragments are incorporated into the genome through homologous recombination. The efficiency of these methods is limited by nonhomologous end joinin...

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Dettagli Bibliografici
Autori principali: Maruyama, Takeshi, Dougan, Stephanie K, Truttmann, Matthias C, Bilate, Angelina M, Ingram, Jessica R, Ploegh, Hidde
Altri autori: Massachusetts Institute of Technology. Department of Biology
Natura: Articolo
Lingua:en_US
Pubblicazione: Nature Publishing Group 2017
Accesso online:http://hdl.handle.net/1721.1/106223
https://orcid.org/0000-0002-1090-6071