Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy

We recently engineered encodable lanthanide binding tags (LBTs) into proteins and demonstrated their applicability in Nuclear Magnetic Resonance (NMR) spectroscopy, X-ray crystallography and luminescence studies. Here, we engineered two-loop-LBTs into the model protein interleukin-1β (IL1β) and meas...

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Bibliographic Details
Main Authors: Barthelmes, Dominic, Gränz, Markus, Barthelmes, Katja, Allen, Karen N., Imperiali, Barbara, Prisner, Thomas, Schwalbe, Harald
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:English
Published: Springer Netherlands 2017
Online Access:http://hdl.handle.net/1721.1/106575
https://orcid.org/0000-0002-5749-7869