A Multi-RNAi Microsponge Platform for Simultaneous Controlled Delivery of Multiple Small Interfering RNAs
Packaging multiple small interfering RNA (siRNA) molecules into nanostructures at precisely defined ratios is a powerful delivery strategy for effective RNA interference (RNAi) therapy. We present a novel RNA nanotechnology based approach to produce multiple components of polymerized siRNA molecules...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Wiley Blackwell
2017
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| Online Access: | http://hdl.handle.net/1721.1/106977 https://orcid.org/0000-0002-4954-8443 https://orcid.org/0000-0003-3988-0837 |
| _version_ | 1811080902712754176 |
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| author | Park, Jae Hyon Roh, Young Hoon Dreaden, Erik Yun, Dong Soo Shopsowitz, Kevin Hammond, Paula T Deng, Zhou J. |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Park, Jae Hyon Roh, Young Hoon Dreaden, Erik Yun, Dong Soo Shopsowitz, Kevin Hammond, Paula T Deng, Zhou J. |
| author_sort | Park, Jae Hyon |
| collection | MIT |
| description | Packaging multiple small interfering RNA (siRNA) molecules into nanostructures at precisely defined ratios is a powerful delivery strategy for effective RNA interference (RNAi) therapy. We present a novel RNA nanotechnology based approach to produce multiple components of polymerized siRNA molecules that are simultaneously self-assembled and densely packaged into composite sponge-like porous microstructures (Multi-RNAi-MSs) by rolling circle transcription. The Multi-RNAi-MSs were designed to contain a combination of multiple polymeric siRNA molecules with precisely controlled stoichiometry within a singular microstructure by manipulating the types and ratios of the circular DNA templates. The Multi-RNAi-MSs were converted into nanosized complexes by polyelectrolyte condensation to manipulate their physicochemical properties (size, shape, and surface charge) for favorable delivery, while maintaining the multifunctional properties of the siRNAs for combined therapeutic effects. These Multi-RNAi-MS systems have great potential in RNAi-mediated biomedical applications, for example, for the treatment of cancer, genetic disorders, and viral infections. |
| first_indexed | 2024-09-23T11:38:36Z |
| format | Article |
| id | mit-1721.1/106977 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:38:36Z |
| publishDate | 2017 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/1069772022-09-27T20:58:10Z A Multi-RNAi Microsponge Platform for Simultaneous Controlled Delivery of Multiple Small Interfering RNAs Park, Jae Hyon Roh, Young Hoon Dreaden, Erik Yun, Dong Soo Shopsowitz, Kevin Hammond, Paula T Deng, Zhou J. Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Roh, Young Hoon Deng, Zhou Dreaden, Erik Yun, Dong Soo Shopsowitz, Kevin Hammond, Paula T Packaging multiple small interfering RNA (siRNA) molecules into nanostructures at precisely defined ratios is a powerful delivery strategy for effective RNA interference (RNAi) therapy. We present a novel RNA nanotechnology based approach to produce multiple components of polymerized siRNA molecules that are simultaneously self-assembled and densely packaged into composite sponge-like porous microstructures (Multi-RNAi-MSs) by rolling circle transcription. The Multi-RNAi-MSs were designed to contain a combination of multiple polymeric siRNA molecules with precisely controlled stoichiometry within a singular microstructure by manipulating the types and ratios of the circular DNA templates. The Multi-RNAi-MSs were converted into nanosized complexes by polyelectrolyte condensation to manipulate their physicochemical properties (size, shape, and surface charge) for favorable delivery, while maintaining the multifunctional properties of the siRNAs for combined therapeutic effects. These Multi-RNAi-MS systems have great potential in RNAi-mediated biomedical applications, for example, for the treatment of cancer, genetic disorders, and viral infections. United States. Department of Defense. Ovarian Cancer Research Program (Teal Innovator Award OC120504) National Research Foundation of Korea (Grant 2015R1C1A1A02037770) Yonsei University (Future-leading Research Initiative 2015-22-0085) National Institutes of Health (U.S.) (Grant 1F32EB017614-02) 2017-02-16T20:15:34Z 2017-02-16T20:15:34Z 2015-12 2015-11 Article http://purl.org/eprint/type/JournalArticle 1433-7851 1521-3773 http://hdl.handle.net/1721.1/106977 Roh, Young Hoon et al. “A Multi-RNAi Microsponge Platform for Simultaneous Controlled Delivery of Multiple Small Interfering RNAs.” Angewandte Chemie International Edition 55.10 (2016): 3347–3351. https://orcid.org/0000-0002-4954-8443 https://orcid.org/0000-0003-3988-0837 en_US http://dx.doi.org/10.1002/anie.201508978 Angewandte Chemie International Edition Creative Commons Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ application/pdf Wiley Blackwell Wiley |
| spellingShingle | Park, Jae Hyon Roh, Young Hoon Dreaden, Erik Yun, Dong Soo Shopsowitz, Kevin Hammond, Paula T Deng, Zhou J. A Multi-RNAi Microsponge Platform for Simultaneous Controlled Delivery of Multiple Small Interfering RNAs |
| title | A Multi-RNAi Microsponge Platform for Simultaneous Controlled Delivery of Multiple Small Interfering RNAs |
| title_full | A Multi-RNAi Microsponge Platform for Simultaneous Controlled Delivery of Multiple Small Interfering RNAs |
| title_fullStr | A Multi-RNAi Microsponge Platform for Simultaneous Controlled Delivery of Multiple Small Interfering RNAs |
| title_full_unstemmed | A Multi-RNAi Microsponge Platform for Simultaneous Controlled Delivery of Multiple Small Interfering RNAs |
| title_short | A Multi-RNAi Microsponge Platform for Simultaneous Controlled Delivery of Multiple Small Interfering RNAs |
| title_sort | multi rnai microsponge platform for simultaneous controlled delivery of multiple small interfering rnas |
| url | http://hdl.handle.net/1721.1/106977 https://orcid.org/0000-0002-4954-8443 https://orcid.org/0000-0003-3988-0837 |
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