Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery
Synthetic regulatory circuits encoded in RNA rather than DNA could provide a means to control cell behavior while avoiding potentially harmful genomic integration in therapeutic applications. We create post-transcriptional circuits using RNA-binding proteins, which can be wired in a plug-and-play fa...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2017
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| Online Access: | http://hdl.handle.net/1721.1/107113 https://orcid.org/0000-0003-2265-3363 https://orcid.org/0000-0001-7734-9153 https://orcid.org/0000-0002-0302-6834 https://orcid.org/0000-0003-0396-2443 |
| _version_ | 1811090817379467264 |
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| author | Endo, Kei Saito, Hirohide Wroblewska, Liliana Kitada, Tasuku Siciliano, Velia DiAndreth, Breanna Elizabeth Weiss, Ron |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Endo, Kei Saito, Hirohide Wroblewska, Liliana Kitada, Tasuku Siciliano, Velia DiAndreth, Breanna Elizabeth Weiss, Ron |
| author_sort | Endo, Kei |
| collection | MIT |
| description | Synthetic regulatory circuits encoded in RNA rather than DNA could provide a means to control cell behavior while avoiding potentially harmful genomic integration in therapeutic applications. We create post-transcriptional circuits using RNA-binding proteins, which can be wired in a plug-and-play fashion to create networks of higher complexity. We show that the circuits function in mammalian cells when encoded in modified mRNA or self-replicating RNA. |
| first_indexed | 2024-09-23T14:52:25Z |
| format | Article |
| id | mit-1721.1/107113 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:52:25Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1071132022-10-01T23:04:04Z Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery Endo, Kei Saito, Hirohide Wroblewska, Liliana Kitada, Tasuku Siciliano, Velia DiAndreth, Breanna Elizabeth Weiss, Ron Massachusetts Institute of Technology. Department of Biological Engineering Wroblewska, Liliana Kitada, Tasuku Siciliano, Velia DiAndreth, Breanna Elizabeth Weiss, Ron Synthetic regulatory circuits encoded in RNA rather than DNA could provide a means to control cell behavior while avoiding potentially harmful genomic integration in therapeutic applications. We create post-transcriptional circuits using RNA-binding proteins, which can be wired in a plug-and-play fashion to create networks of higher complexity. We show that the circuits function in mammalian cells when encoded in modified mRNA or self-replicating RNA. United States. Defense Advanced Research Projects Agency (Grant DARPA-BAA-11-23) National Institutes of Health (U.S.) (Grants P50-GM098792 and 5-R01-CA155320-03) National Science Foundation (U.S.). Graduate Research Fellowship Program (Grant 1122374) 2017-02-23T14:54:14Z 2017-02-23T14:54:14Z 2015-08 2015-04 Article http://purl.org/eprint/type/JournalArticle 1087-0156 1546-1696 http://hdl.handle.net/1721.1/107113 Wroblewska, Liliana et al. “Mammalian Synthetic Circuits with RNA Binding Proteins for RNA-Only Delivery.” Nature Biotechnology 33.8 (2015): 839–841. https://orcid.org/0000-0003-2265-3363 https://orcid.org/0000-0001-7734-9153 https://orcid.org/0000-0002-0302-6834 https://orcid.org/0000-0003-0396-2443 en_US http://dx.doi.org/10.1038/nbt.3301 Nature Biotechnology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Endo, Kei Saito, Hirohide Wroblewska, Liliana Kitada, Tasuku Siciliano, Velia DiAndreth, Breanna Elizabeth Weiss, Ron Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery |
| title | Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery |
| title_full | Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery |
| title_fullStr | Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery |
| title_full_unstemmed | Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery |
| title_short | Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery |
| title_sort | mammalian synthetic circuits with rna binding proteins for rna only delivery |
| url | http://hdl.handle.net/1721.1/107113 https://orcid.org/0000-0003-2265-3363 https://orcid.org/0000-0001-7734-9153 https://orcid.org/0000-0002-0302-6834 https://orcid.org/0000-0003-0396-2443 |
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