Virtual microfluidics for digital quantification and single-cell sequencing

We have developed hydrogel-based virtual microfluidics as a simple and robust alternative to complex engineered microfluidic systems for the compartmentalization of nucleic acid amplification reactions. We applied in-gel digital multiple displacement amplification (dMDA) to purified DNA templates, c...

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Bibliographic Details
Main Authors: Xu, Liyi, Brito, Ilana Lauren, Alm, Eric J, Blainey, Paul C
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:en_US
Published: Nature Publishing Group 2017
Online Access:http://hdl.handle.net/1721.1/107124
https://orcid.org/0000-0002-6699-2634
https://orcid.org/0000-0001-8294-9364
https://orcid.org/0000-0001-7014-3830