Virtual microfluidics for digital quantification and single-cell sequencing
We have developed hydrogel-based virtual microfluidics as a simple and robust alternative to complex engineered microfluidic systems for the compartmentalization of nucleic acid amplification reactions. We applied in-gel digital multiple displacement amplification (dMDA) to purified DNA templates, c...
Main Authors: | Xu, Liyi, Brito, Ilana Lauren, Alm, Eric J, Blainey, Paul C |
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Other Authors: | Massachusetts Institute of Technology. Department of Biological Engineering |
Format: | Article |
Language: | en_US |
Published: |
Nature Publishing Group
2017
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Online Access: | http://hdl.handle.net/1721.1/107124 https://orcid.org/0000-0002-6699-2634 https://orcid.org/0000-0001-8294-9364 https://orcid.org/0000-0001-7014-3830 |
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