Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells
The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/H3K27me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2017
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| Online Access: | http://hdl.handle.net/1721.1/107488 |
| _version_ | 1826194732553863168 |
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| author | Juan, Aster H. Wang, Stan Ko, Kyung Dae Zare, Hossein Tsai, Pei-Fang Feng, Xuesong Ascoli, Anthony M. Gutierrez-Cruz, Gustavo Krebs, Jordan Sidoli, Simone Knight, Adam L. Pedersen, Roger A. Garcia, Benjamin A. Casellas, Rafael Zou, Jizhong Sartorelli, Vittorio Vivanco, Karinna O. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Juan, Aster H. Wang, Stan Ko, Kyung Dae Zare, Hossein Tsai, Pei-Fang Feng, Xuesong Ascoli, Anthony M. Gutierrez-Cruz, Gustavo Krebs, Jordan Sidoli, Simone Knight, Adam L. Pedersen, Roger A. Garcia, Benjamin A. Casellas, Rafael Zou, Jizhong Sartorelli, Vittorio Vivanco, Karinna O. |
| author_sort | Juan, Aster H. |
| collection | MIT |
| description | The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/H3K27me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESCs) with a modified H3K27me2/H3K27me3 ratio. Our findings document dynamic developmental control in the genomic distribution of H3K27me2 and H3K27me3 at regulatory regions in ESCs. They also reveal that modifying the ratio of H3K27me2 and H3K27me3 is sufficient for the acquisition and repression of defined cell lineage transcriptional programs and phenotypes and influences induction of the ESC ground state. |
| first_indexed | 2024-09-23T10:01:16Z |
| format | Article |
| id | mit-1721.1/107488 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:01:16Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1074882022-09-30T18:21:00Z Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells Juan, Aster H. Wang, Stan Ko, Kyung Dae Zare, Hossein Tsai, Pei-Fang Feng, Xuesong Ascoli, Anthony M. Gutierrez-Cruz, Gustavo Krebs, Jordan Sidoli, Simone Knight, Adam L. Pedersen, Roger A. Garcia, Benjamin A. Casellas, Rafael Zou, Jizhong Sartorelli, Vittorio Vivanco, Karinna O. Massachusetts Institute of Technology. Department of Biological Engineering Vivanco, Karinna O. The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/H3K27me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESCs) with a modified H3K27me2/H3K27me3 ratio. Our findings document dynamic developmental control in the genomic distribution of H3K27me2 and H3K27me3 at regulatory regions in ESCs. They also reveal that modifying the ratio of H3K27me2 and H3K27me3 is sufficient for the acquisition and repression of defined cell lineage transcriptional programs and phenotypes and influences induction of the ESC ground state. National Institute of Arthritis and Musculoskeletal and Skin Diseases (U.S.). Intramural Research Program 2017-03-20T14:20:57Z 2017-03-20T14:20:57Z 2016-10 2016-09 Article http://purl.org/eprint/type/JournalArticle 2211-1247 http://hdl.handle.net/1721.1/107488 Juan, Aster H. et al. “Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells.” Cell Reports 17.5 (2016): 1369–1382. © 2017 Elsevier en_US http://dx.doi.org/10.1016/j.celrep.2016.09.087 Cell Reports Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier Elsevier |
| spellingShingle | Juan, Aster H. Wang, Stan Ko, Kyung Dae Zare, Hossein Tsai, Pei-Fang Feng, Xuesong Ascoli, Anthony M. Gutierrez-Cruz, Gustavo Krebs, Jordan Sidoli, Simone Knight, Adam L. Pedersen, Roger A. Garcia, Benjamin A. Casellas, Rafael Zou, Jizhong Sartorelli, Vittorio Vivanco, Karinna O. Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells |
| title | Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells |
| title_full | Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells |
| title_fullStr | Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells |
| title_full_unstemmed | Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells |
| title_short | Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells |
| title_sort | roles of h3k27me2 and h3k27me3 examined during fate specification of embryonic stem cells |
| url | http://hdl.handle.net/1721.1/107488 |
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