Transcription factor trapping by RNA in gene regulatory elements
Transcription factors (TFs) bind specific sequences in promoter-proximal and -distal DNA elements to regulate gene transcription. RNA is transcribed from both of these DNA elements, and some DNA binding TFs bind RNA. Hence, RNA transcribed from regulatory elements may contribute to stable TF occupan...
| मुख्य लेखकों: | , , , , , , , , , |
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| अन्य लेखक: | |
| स्वरूप: | लेख |
| भाषा: | en_US |
| प्रकाशित: |
American Association for the Advancement of Science (AAAS)
2017
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| ऑनलाइन पहुंच: | http://hdl.handle.net/1721.1/108470 https://orcid.org/0000-0003-1465-1691 https://orcid.org/0000-0001-8855-8647 |
| सारांश: | Transcription factors (TFs) bind specific sequences in promoter-proximal and -distal DNA elements to regulate gene transcription. RNA is transcribed from both of these DNA elements, and some DNA binding TFs bind RNA. Hence, RNA transcribed from regulatory elements may contribute to stable TF occupancy at these sites. We show that the ubiquitously expressed TF Yin-Yang 1 (YY1) binds to both gene regulatory elements and their associated RNA species across the entire genome. Reduced transcription of regulatory elements diminishes YY1 occupancy, whereas artificial tethering of RNA enhances YY1 occupancy at these elements. We propose that RNA makes a modest but important contribution to the maintenance of certain TFs at gene regulatory elements and suggest that transcription of regulatory elements produces a positive-feedback loop that contributes to the stability of gene expression programs. |
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