Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies

Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies

Expansion microscopy (ExM) enables imaging of preserved specimens with nanoscale precision on diffraction-limited instead of specialized super-resolution microscopes. ExM works by physically separating fluorescent probes after anchoring them to a swellable gel. The first ExM method did not result in...

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Bibliographic Details
Main Authors: English, Brian P, Gao, Linyi, Suk, Ho-Jun, Yoshida, Fumiaki, DeGennaro, Ellen M, Roossien, Douglas H, Cai, Dawen, Tillberg, Paul W., Chen, Fei, Piatkevich, Kiryl, Zhao, Yongxin, Yu, Chih-Chieh, Martorell, Anthony, Gong, Guanyu, Seneviratne, Uthpala Indrajith, Tannenbaum, Steven R, Desimone, Robert, Boyden, Edward
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:en_US
Published: Nature Publishing Group 2017
Online Access:http://hdl.handle.net/1721.1/108514
https://orcid.org/0000-0003-0254-4741
https://orcid.org/0000-0003-3776-4605
https://orcid.org/0000-0003-4188-5725
https://orcid.org/0000-0001-8713-0446
https://orcid.org/0000-0002-3579-0327
https://orcid.org/0000-0002-2206-2590
https://orcid.org/0000-0002-0899-6709
https://orcid.org/0000-0001-6774-9639
https://orcid.org/0000-0002-5938-4227
https://orcid.org/0000-0002-0419-3351

Internet

http://hdl.handle.net/1721.1/108514
https://orcid.org/0000-0003-0254-4741
https://orcid.org/0000-0003-3776-4605
https://orcid.org/0000-0003-4188-5725
https://orcid.org/0000-0001-8713-0446
https://orcid.org/0000-0002-3579-0327
https://orcid.org/0000-0002-2206-2590
https://orcid.org/0000-0002-0899-6709
https://orcid.org/0000-0001-6774-9639
https://orcid.org/0000-0002-5938-4227
https://orcid.org/0000-0002-0419-3351

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