Fluorophore-Conjugated Holliday Junctions for Generating Super-Bright Antibodies and Antibody Fragments

Fluorophore-Conjugated Holliday Junctions for Generating Super-Bright Antibodies and Antibody Fragments

The site-specific modification of proteins with fluorophores can render a protein fluorescent without compromising its function. To avoid self-quenching from multiple fluorophores installed in close proximity, we used Holliday junctions to label proteins site-specifically. Holliday junctions enable...

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Bibliographic Details
Main Authors: Theile, Christopher S., Chen, Guan-Yu, Bilate, Angelina M., Duarte, Joao N., Avalos, Ana M., Fang, Tao, Barberena, Roberto, Sato, Shuji, Ploegh, Hidde, Li, Zeyang,S.M.Massachusetts Institute of Technology.
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:en_US
Published: Wiley Blackwell 2017
Online Access:http://hdl.handle.net/1721.1/108551
https://orcid.org/0000-0002-1090-6071

Internet

http://hdl.handle.net/1721.1/108551
https://orcid.org/0000-0002-1090-6071

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