Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

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Transpeptidation catalyzed by ​sortase A allows the preparation of proteins that are site-specifically and homogeneously modified with a wide variety of functional groups, such as fluorophores, PEG moieties, lipids, glycans, bio-orthogonal reactive groups and affinity handles. This protocol describe...

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Bibliographic Details
Main Authors: Witte, Martin D, Wu, Tongfei, Guimaraes, Carla P, Theile, Christopher S, Blom, Annet E M, Ingram, Jessica R, Kundrat, Lenka, Goldberg, Shalom D, Ploegh, Hidde, Li, Zeyang,S.M.Massachusetts Institute of Technology.
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:en_US
Published: Nature Publishing Group 2017
Online Access:http://hdl.handle.net/1721.1/109371
https://orcid.org/0000-0002-1090-6071

Internet

http://hdl.handle.net/1721.1/109371
https://orcid.org/0000-0002-1090-6071

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